Výsledky vyhledávání - "Reuben Leberman"

Analysis and overexpression in Escherichia coli of a staphylococcal gene encoding seryl-tRNA synthetase

Autor: Michael Härtlein, Laurence Seignovert, Reuben Leberman, Mélanie Beaulande, Niko Bausch

Publikováno v: Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1397:169-174

We have sequenced and expressed in Escherichia coli the gene encoding the seryl-tRNA synthetase from the pathogenic bacterium Staphylococcus aureus. The overexpressed and purified recombinant enzyme was able to aminoacylate unfractionated tRNA from E

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff82b91861b7d3e1c712f5ab910e5e5d
https://doi.org/10.1016/s0167-4781(98)00027-x

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Seryl-tRNA Synthetase from the Extreme Halophile Haloarcula marismortui. Isolation, Characterization and Sequencing of the Gene and its Expression in Escherichia coli

Autor: Michael Härtlein, Claire M.-J. Taupin, Reuben Leberman

Publikováno v: European Journal of Biochemistry. 243:141-150

The seryl-tRNA synthetase from the extreme halophilic archaebacterium Haloarcula marismortul, belonging to the group Euryarchaeota, has been purified and its hyperhalophilic behavior demonstrated by activity and stability tests in KCl, NaCl and MgCl2

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0059280b1e2284dbff826ef436aa866a
https://doi.org/10.1111/j.1432-1033.1997.0141a.x

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Isolation and characterization of an Escherichia coli seryl-tRNA synthetase mutant with a large increase in Km for serine

Autor: Michael Härtlein, J C Willison, Reuben Leberman

Publikováno v: Journal of Bacteriology. 177:3347-3350

A mutant of Escherichia coli resistant to serine hydroxamate which has a large increase in Km for serine of seryl-tRNA synthetase is described. The mutant serS gene was cloned and sequenced and was found to contain a single-base-pair mutation, result

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::573cff8ef6937ba8fec2658403999a3e
https://doi.org/10.1128/jb.177.11.3347-3350.1995

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Seryl-tRNA synthetase fromEscherichia coli: functional evidence for cross-dimer tRNA binding during aminoacylation

Autor: Michael Härtlein, J C Willison, Reuben Leberman, Franck Borel, Christine Vincent

Publikováno v: Nucleic Acids Research. 23:1113-1118

Escherichia coli seryl-tRNA synthetase (SerRS) is a homo-dimeric class II aminoacyl-tRNA synthetase. Each subunit is composed of two distinct domains: the N-terminal domain is a 60 A long, arm-like coiled coil structure built up of two antiparallel a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89477d69f0542fe4b8bf6d7ac48a3338
https://doi.org/10.1093/nar/23.7.1113

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Crystal Structures at 2.5 Angstrom Resolution of Seryl-tRNA Synthetase Complexed with Two Analogs of Seryl Adenylate

Autor: Michael Tukalo, Carmen Berthet-Colominas, Stephen Cusack, Mogens S. Lehmann, Gerhard Grübel, Hassan Belrhali, Anna Yaremchuk, Reuben Leberman, Barbro Beijer, Jean-Francois Legrand, Kjeld Larsen, Brian S. Sproat, Jens Aage Als-Nielsen

Publikováno v: Science. 263:1432-1436

Crystal structures of seryl-tRNA synthetase from Thermus thermophilus complexed with two different analogs of seryl adenylate have been determined at 2.5 A resolution. The first complex is between the enzyme and seryl-hydroxamate-AMP (adenosine monop

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bb0ec19643bf81c358084056cd318ab4
https://doi.org/10.1126/science.8128224

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Seryl-tRNA synthetase fromEscherichia coli: implication of its N-terminal domain in aminoacylation activity and specificity

Autor: Christine Vincent, Franck Borel, Michael Härtlein, Reuben Leberman

Publikováno v: Nucleic Acids Research. 22:2963-2969

Escherichia coli seryl-tRNA synthetase (SerRS) a dimeric class II aminoacyl-tRNA synthetase with two structural domains charges specifically the five iso-acceptor tRNA(ser) as well as the tRNA(sec) (selC product) of E. coli. The N-terminal domain is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fca09275f54d9aff81134b45c4a631fa
https://doi.org/10.1093/nar/22.15.2963

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In vivo overexpression and purification ofEscherichia colitRNAser

Autor: Michael Härtlein, Franck Borel, Reuben Leberman

Publikováno v: FEBS Letters. 324:162-166

DNA fragments corresponding to the sequences of Escherichia coli tRNA(2ser) and amber suppressor tRNA(ser), were synthesized from overlapping oligonucleotides. These were interposed between a strong promotor and a synthetic transcriptional terminator

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8aab35cb405c1346b63fefa763a594a5
https://doi.org/10.1016/0014-5793(93)81385-d

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Optimizing protein crystallization by aggregate size distribution analysis using dynamic light scattering

Autor: Jo¨rg Langowski, Reuben Leberman, Francois Thibault

Publikováno v: Journal of Crystal Growth. 122:50-59

Dynamic light scattering (DLS) techniques have been used on several systems to distinguish between amorphous and crystalline aggregation of proteins during precipitation. Most of these studies suffer from either of two defects: (i) they have been con

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::352d23aacd95e7c7977f9b7705403301
https://doi.org/10.1016/0022-0248(92)90225-8

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