Zobrazeno 1 - 10
of 33 084
pro vyhledávání: '"Reporter gene"'
Publikováno v:
陆军军医大学学报, Vol 46, Iss 17, Pp 1959-1968 (2024)
Objective To investigate the feasibility of granzyme B (GB) promoter-controlled ferritin heavy chain (FTH1) reporter gene expression for monitoring the activation status of chimeric antigen receptor T cells (CAR-T) by magnetic resonance imaging (MRI)
Externí odkaz:
https://doaj.org/article/b92548c2f9ce47429828cc255be42a20
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-9 (2024)
Abstract To investigate the impact of different 5ʹ untranslated regions (UTRs) on mRNA vaccine translation efficiency, five dual-reporter gene expression plasmids with different 5ʹUTRs were constructed. The corresponding mRNA transcripts were trans
Externí odkaz:
https://doaj.org/article/bdbd06cc39374b3f83ecbac8671ec356
Autor:
Austin D.C. Miller, Soham P. Chowdhury, Hadley W. Hanson, Sarah K. Linderman, Hannah I. Ghasemi, Wyatt D. Miller, Meghan A. Morrissey, Chris D. Richardson, Brooke M. Gardner, Arnab Mukherjee
Publikováno v:
Journal of Biological Engineering, Vol 18, Iss 1, Pp 1-14 (2024)
Abstract Aquaporin-1 (Aqp1), a water channel, has garnered significant interest for cell-based medicine and in vivo synthetic biology due to its ability to be genetically encoded to produce magnetic resonance signals by increasing the rate of water d
Externí odkaz:
https://doaj.org/article/b290fa163a9e4789a667fe3c07ab057a
Autor:
E. A. Khaova, A. G. Tkachenko
Publikováno v:
Вавиловский журнал генетики и селекции, Vol 28, Iss 1, Pp 24-32 (2024)
Polyamines and indole are small regulatory molecules that are involved in the adaptation to stress in bacteria, including the regulation of gene expression. Genes, the translation of which is under the regulatory effects of polyamines, form the polya
Externí odkaz:
https://doaj.org/article/0c097761d526434cb7eb47c57e7c1f3f
Publikováno v:
Nepal Journal of Biotechnology, Vol 12, Iss 1 (2024)
The field of genetic engineering and molecular breeding utilizes maize immature embryos for transformation studies. However, there are hinderances such as the unavailability of immature embryos throughout the year and their low transformation frequen
Externí odkaz:
https://doaj.org/article/07d7ec61a58a4caf90061ea66d567a1b
Autor:
Nina Lautenschläger, Katja Schmidt, Carolin Schiffer, Thomas F. Wulff, Karin Hahnke, Knut Finstermeier, Moïse Mansour, Alexander K. W. Elsholz, Emmanuelle Charpentier
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Genetic tools form the basis for the study of molecular mechanisms. Despite many recent advances in the field of genetic engineering in bacteria, genetic toolsets remain scarce for non-model organisms, such as the obligatory human pathogen Streptococ
Externí odkaz:
https://doaj.org/article/ab38edfc233547cf9549c6c011dab4ff
Autor:
Lucas Pedraz, Eduard Torrents
Publikováno v:
BioTechniques, Vol 75, Iss 6, Pp 250-255 (2023)
Fluorescent proteins, such as green fluorescent proteins, are invaluable tools for detecting and quantifying gene expression in high-throughput reporter gene assays. However, they introduce significant inaccuracies in studies involving microaerobiosi
Externí odkaz:
https://doaj.org/article/c1880ed8925b4deea42e6f9f93c313c0
Autor:
Van Giap Do, Seonae Kim, Nay Myo Win, Soon-Il Kwon, Hunjoong Kweon, Sangjin Yang, Juhyeon Park, Gyungran Do, Youngsuk Lee
Publikováno v:
Plants, Vol 13, Iss 19, p 2803 (2024)
Genetic transformation is a critical tool for gene manipulation and functional analyses in plants, enabling the exploration of key phenotypes and agronomic traits at the genetic level. While dicotyledonous plants offer various tissues for in vitro cu
Externí odkaz:
https://doaj.org/article/d495d0b4e12645b69430a09ed64373ae
Autor:
Sergio Utrilla-Trigo, Luis Jiménez-Cabello, Alejandro Marín-López, Miguel Illescas-Amo, Germán Andrés, Eva Calvo-Pinilla, Gema Lorenzo, Piet A. van Rijn, Javier Ortego, Aitor Nogales
Publikováno v:
Microbiology Spectrum, Vol 12, Iss 3 (2024)
ABSTRACTBluetongue virus (BTV) is the causative agent of the important livestock disease bluetongue (BT), which is transmitted via Culicoides bites. BT causes severe economic losses associated with its considerable impact on health and trade of anima
Externí odkaz:
https://doaj.org/article/3ca694724c584e07b9f8d6ac01d0092c