Zobrazeno 1 - 10
of 18
pro vyhledávání: '"René Verwaal"'
Autor:
Tina Fink, Bojana Stevović, René Verwaal, Johannes A. Roubos, Rok Gaber, Mojca Benčina, Roman Jerala, Helena Gradišar
Publikováno v:
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
Abstract The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to increase the biosynthetic produ
Externí odkaz:
https://doaj.org/article/eafc2fc0d72845db91f1a302bce6ded8
Publikováno v:
Ciurkot, K W, Gorochowski, T E, Roubos, J A & Verwaal, R 2021, ' Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a ', Nucleic Acids Research, vol. 49, no. 13, gkab529, pp. 7775-7790 . https://doi.org/10.1093/nar/gkab529
Nucleic Acids Research
Nucleic Acids Research
CRISPR Cas12a is an RNA-programmable endonuclease particularly suitable for gene regulation. This is due to its preference for T-rich PAMs that allows it to more easily target AT-rich promoter sequences, and built-in RNase activity which can process
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c64301b120308cd6c40facea8754c32
https://doi.org/10.1093/nar/gkab529
https://doi.org/10.1093/nar/gkab529
Autor:
Bojana Stevović, Roman Jerala, Helena Gradišar, Johannes Andries Roubos, Tina Fink, Mojca Benčina, Rok Gaber, René Verwaal
Publikováno v:
AMB Express
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
AMB Express, Vol 10, Iss 1, Pp 1-10 (2020)
The clustering of biosynthetic enzymes is used in nature to channel reaction products and increase the yield of compounds produced by multiple reaction steps. The coupling of multiple enzymes has been shown to increase the biosynthetic product yield.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af79270b2c7de199f2a096926971e363
https://doi.org/10.21203/rs.3.rs-21832/v2
https://doi.org/10.21203/rs.3.rs-21832/v2
Publikováno v:
Yeast. 35:201-211
Cpf1 represents a novel single RNA-guided CRISPR/Cas endonuclease system suitable for genome editing with distinct features compared with Cas9. We demonstrate the functionality of three Cpf1 orthologues - Acidaminococcus spp. BV3L6 (AsCpf1), Lachnosp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e80d603ee11768f2189ad0632546a6c8
https://doi.org/10.1002/yea.3278
https://doi.org/10.1002/yea.3278
Publikováno v:
Journal of visualized experiments : JoVE. (147)
High efficiency, ease of use and versatility of the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has facilitated advanced genetic modification of Saccharomyces cerevisiae, a model organism
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cc8d9f3d57948ba6d8aba7245dc8d336
https://pubmed.ncbi.nlm.nih.gov/31205318
https://pubmed.ncbi.nlm.nih.gov/31205318
Autor:
John van der Oost, Johannes Andries Roubos, René Verwaal, Belén Adiego-Pérez, Pascale Daran-Lapujade, Paola Randazzo, Jean-Marc Daran
Publikováno v:
FEMS Microbiology Letters 366 (2019) 8
FEMS Microbiology Letters
FEMS Microbiology Letters, 366(8)
FEMS Microbiology Letters
FEMS Microbiology Letters, 366(8)
Microbial production of chemical compounds often requires highly engineered microbial cell factories. During the last years, CRISPR-Cas nucleases have been repurposed as powerful tools for genome editing. Here, we briefly review the most frequently u
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b98ea60f536fe742a623d1ebee2dc6d
https://doi.org/10.1093/femsle/fnz086
https://doi.org/10.1093/femsle/fnz086
Publikováno v:
Yeast
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=r3c4b2081b22::e80d603ee11768f2189ad0632546a6c8
Publikováno v:
Yeast (Chichester, England)
Cpf1 represents a novel single RNA‐guided CRISPR/Cas endonuclease system suitable for genome editing with distinct features compared with Cas9. We demonstrate the functionality of three Cpf1 orthologues – Acidaminococcus spp. BV3L6 (AsCpf1), Lach
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::0f71bb6396d7cf10ed21367e27d64799
https://pubmed.ncbi.nlm.nih.gov/28886218
https://pubmed.ncbi.nlm.nih.gov/28886218
Publikováno v:
Yeast. 21:747-757
Hexose transporter (Hxt) proteins transport glucose across the plasma membrane in the yeast Saccharomyces cerevisiae. Recently, we have shown that expression of HXT5 is regulated by the growth rate of the cells. Because gene expression is regulated b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::269ff78dfdfd7c7129f5c61eef484280
https://doi.org/10.1002/yea.1106
https://doi.org/10.1002/yea.1106
Autor:
Arie J. Verkleij, C. Theo Verrips, Johannes Boonstra, Sjoukje H. Slofstra, René Verwaal, Johannes W. G. Paalman
Publikováno v:
FEMS Yeast Research. 3:261-268
Several factors may control trehalose and glycogen synthesis, like the glucose flux, the growth rate, the intracellular glucose-6-phosphate level and the glucose concentration in the medium. Here, the possible relation of these putative inducers to r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::50d958381b29512e5cc711b5c467a12c
https://doi.org/10.1111/j.1567-1364.2003.tb00168.x
https://doi.org/10.1111/j.1567-1364.2003.tb00168.x