Zobrazeno 1 - 7
of 7
pro vyhledávání: '"René Daer"'
Publikováno v:
APL Bioengineering, Vol 4, Iss 1, Pp 016102-016102-8 (2020)
Previously, we used an inducible, transgenic polycomb chromatin system to demonstrate that closed, transcriptionally silenced chromatin reduces Cas9 editing. Here, we investigated strategies to enhance Cas9 editing efficiency by artificially perturbi
Externí odkaz:
https://doaj.org/article/8ff761d22bb049789368acb10e414e6e
Autor:
René Daer, Cassandra M Barrett, Ernesto Luna Melendez, Jiaqi Wu, Stefan J Tekel, Jimmy Xu, Brady Dennison, Ryan Muller, Karmella A Haynes
Publikováno v:
PLoS ONE, Vol 13, Iss 8, p e0202294 (2018)
Quorum sensing networks have been identified in over one hundred bacterial species to date. A subset of these networks regulate group behaviors, such as bioluminescence, virulence, and biofilm formation, by sending and receiving small molecules calle
Externí odkaz:
https://doaj.org/article/603be5ecbd2f49b0afe01fe57ba46a2e
Autor:
Kaushal Rege, Matthew D. Christensen, Sheba Goklany, Karmella A. Haynes, Seyedehmelika Meraji, René Daer, Sudhakar Godeshala, Rajeshwar Nitiyanandan
Publikováno v:
J Control Release
Effective transgene expression in mammalian cells relies on successful delivery, cytoplasmic trafficking, and nuclear translocation of the delivered vector, but delivery is impeded by several formidable physicochemical barriers on the surface of and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbcbb3fb088017fee77dcc52ed71ba1f
https://doi.org/10.1016/j.jconrel.2018.06.030
https://doi.org/10.1016/j.jconrel.2018.06.030
Autor:
Karmella A. Haynes, Brady Dennison, René Daer, Cassandra M Barrett, Jimmy Xu, Ernesto Luna Melendez, Jiaqi Wu, Stefan J. Tekel, Ryan Yue Muller
Publikováno v:
PLoS ONE
PLoS ONE, Vol 13, Iss 8, p e0202294 (2018)
PLoS ONE, Vol 13, Iss 8, p e0202294 (2018)
Quorum sensing networks have been identified in over one hundred bacterial species to date. A subset of these networks regulate group behaviors, such as bioluminescence, virulence, and biofilm formation, by sending and receiving small molecules calle
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5acbe485d66675b1fd8a13c1165ef86
https://pubmed.ncbi.nlm.nih.gov/30138364
https://pubmed.ncbi.nlm.nih.gov/30138364
Recently we demonstrated that closed chromatin composed of Polycomb proteins and histone 3 lysine 27 trimethylation impedes CRISPR-mediated genome editing by blocking the accessibility of chromosomal DNA to spCas9/sgRNA. Editing efficiencies were hig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03701b2aab6b1badb4c607bc1886db84
Publikováno v:
npj Genomic Medicine, Vol 2, Iss 1, Pp 1-10 (2017)
Chromatin proteins have expanded the mammalian synthetic biology toolbox by enabling control of active and silenced states at endogenous genes. Others have reported synthetic proteins that bind DNA and regulate genes by altering chromatin marks, such
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d483359d59c92785a497eb3e389a9592
https://doi.org/10.1038/s41525-016-0002-3
https://doi.org/10.1038/s41525-016-0002-3
In order to efficiently edit eukaryotic genomes, it is critical to test the impact of chromatin dynamics on CRISPR/Cas9 function and develop strategies to adapt the system to eukaryotic contexts. So far, research has extensively characterized the rel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::80b369150151e2c169eb11f0d1878954