In vitro capture and characterization of embryonic rosette-stage pluripotency between naive and primed states

Autor: Emiel van Genderen, Yang Ge, Alex Maas, Hendrik Marks, Joop H. Jansen, Wilfred F. J. van IJcken, Rutger W W Brouwer, Alexander T. den Dekker, Irene Escudero, Dorota Kurek, Jente Stel, René A.M. Dirks, Lucas Verwegen, Miha Modic, Johannes Lehmann, Nicolas C. Rivron, Cindy Eleveld, Micha Drukker, Alex Neagu, Esther B. Baart, Derk ten Berge, Guido van Mierlo

Publikováno v: Nature Cell Biology, 22, 5, pp. 534-545
Nature Cell Biology, 22, 534-545
Nature Cell Biology, 22(5), 534-545. Nature Publishing Group
Nature Cell Biology, 22, 534-545. Nature Research

Following implantation, the naive pluripotent epiblast of the mouse blastocyst generates a rosette, undergoes lumenogenesis and forms the primed pluripotent egg cylinder, which is able to generate the embryonic tissues. How pluripotency progression a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::83230315c9a73bd2f7e49524d9668131
https://hdl.handle.net/2066/227169

Terminal keratinocyte differentiation in vitro is associated with a stable DNA methylome

Autor: Ellen H. van den Bogaard, Hendrik Marks, René A.M. Dirks, Merel A.W. Oortveld, Arie B. Brinkman, Patrick L.J.M. Zeeuwen, Jieqiong Qu, Jos P.H. Smits, Joost Schalkwijk, Huiqing Zhou

Publikováno v: Experimental Dermatology, 30, 8, pp. 1023-1032
Experimental Dermatology, 30, 1023-1032
Experimental Dermatology

The epidermal compartment of the skin is regenerated constantly by proliferation of epidermal keratinocytes. Differentiation of a subset of these keratinocytes allows the epidermis to retain its barrier properties. Regulation of keratinocyte fate—w

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7490b1c38f428033458f7bd78e3d51fb
https://repository.ubn.ru.nl/handle/2066/235970

A plug and play microfluidic platform for standardized sensitive low-input Chromatin Immunoprecipitation

Autor: Peter C. Thomas, Hendrik Marks, René A.M. Dirks, Robert C. Jones, Hendrik G. Stunnenberg

Epigenetic profiling by ChIP-Seq has become a powerful tool for genome-wide identification of regulatory elements, for defining transcriptional regulatory networks and for screening for biomarkers. However, the ChIP-Seq protocol for low-input samples

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cb9b2ae299717262630862ec726a5bf

Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states

Autor: Andras Dinnyes, René A.M. Dirks, Alice Jouneau, Hindrik H. D. Kerstens, Julien Maruotti, Roger A. Pedersen, István Bock, Hendrik Marks, Martijn A. Huynen, Guido van Mierlo, Andreia S. Bernardo, Julianna Kobolák

Publikováno v: Epigenetics and Chromatin
Epigenetics and Chromatin, BioMed Central, 2019, 12 (1), pp.1-21. ⟨10.1186/s13072-019-0259-8⟩
Epigenetics & Chromatin
Epigenetics and Chromatin 1 (12), 1-21. (2019)
Epigenetics & Chromatin, 12, pp. 1-21
Epigenetics and Chromatin, 12(1)
Epigenetics and Chromatin 12 (2019) 1
Epigenetics & Chromatin, Vol 12, Iss 1, Pp 1-21 (2019)
Epigenetics and Chromatin, 2019, 12 (1), pp.1-21. ⟨10.1186/s13072-019-0259-8⟩
Epigenetics & Chromatin, 12, 1-21

Background Genomic imprinting, resulting in parent-of-origin specific gene expression, plays a critical role in mammalian development. Here, we apply allele-specific RNA-seq on isogenic B6D2F1 mice to assay imprinted genes in tissues from early embry

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b6953769f564d0de22461f32cd5ece1
https://zenodo.org/record/2567638

Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements

Autor: Cristina Furlan, Michiel Vermeulen, Jing Wang, Mark Lynch, René A.M. Dirks, Peter C. Thomas, Robert C. Jones, Hendrik Marks

Publikováno v: Nature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
Nature Communications
Nature Communications 10 (2019) 1
Nature Communications, 10
Nature Communications, 10(1)

Essentially all cellular processes are orchestrated by protein-protein interactions (PPIs). In recent years, affinity purification coupled to mass spectrometry (AP-MS) has been the preferred method to identify cellular PPIs. Here we present a microfl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::760ce4e08214987ac9b6c3aacfba08da
https://hdl.handle.net/2066/202828