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Autor:
Teisuke, Takita, Hiro, Sakuma, Ren, Ohashi, Somaye, Nilouyal, Sho, Nemoto, Moeka, Wada, Yuya, Yogo, Kaori, Yasuda, Shinichi, Ikushiro, Toshiyuki, Sakaki, Kiyoshi, Yasukawa
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 86:444-454
CYP105A1 from Streptomyces griseolus converts vitamin D3 to its biologically active form, 1α,25-dihydroxy vitamin D3. R73A/R84A mutation enhanced the 1α- and 25-hydroxylation activity for vitamin D3, while M239A mutation generated the 1α-hydroxyla