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pro vyhledávání: '"Relative fluorescence units"'
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Autor:
Alice M. Banks, Colette J. Whitfield, Steven R. Brown, David A. Fulton, Sarah A. Goodchild, Christopher Grant, John Love, Dennis W. Lendrem, Jonathan E. Fieldsend, Thomas P. Howard
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 20, Iss, Pp 218-229 (2022)
Computational and Structural Biotechnology Journal
Computational and Structural Biotechnology Journal
Graphical abstract
Highlights • Novel cell-free protein synthesis reaction buffer improves performance by 400%. • Enhanced performance is maintained across the synthesis of different proteins. • Protein synthesis performance is robust acro
Highlights • Novel cell-free protein synthesis reaction buffer improves performance by 400%. • Enhanced performance is maintained across the synthesis of different proteins. • Protein synthesis performance is robust acro
Autor:
Felipe Hernández, Daniel Valdés, Claudio Vergara, Alvaro Santibañez, Eva Vallejos-Vidal, Claudia Rioseco, Javiera Quiroz, Javiera Alarcon, Carlos Mateluna, Andrea Mella-Torres, Gaby Gutierrez, Sebastián M. Tapia, Esteban Arenillas, Julio Cartagena, Claudia Torrent, Claudio Acuña-Castillo, Javiera Cayunao, Mónica Imarai, Daniela Barria, Javier Mena, Carlos Barrera-Avalos, Alejandro Undurraga, Maximiliano Figueroa, Felipe E. Reyes-López, Valentina Wong, Ana María Sandino, Roberto Luraschi, Deborah Vargas, Ailen Inostroza-Molina
Publikováno v:
Journal of Medical Virology
Because of the COVID-19 pandemic, many transport kits have been manufactured to preserve and transport nasopharyngeal swab samples (NPSs) from patients. However, there is no information on the performance of the different virus transport media (VTM)
Autor:
Hua Deng
Publikováno v:
International Journal of Plant Biology, Vol 7, Iss 1 (2016)
In this work we conducted a quantitative analysis of the nuclear DNA-content of developing sperm cells of the plant Brassica campestris L. The sperm cells were in young pollen grain, mature pollen grain and pollen tubes. When generative cells, at the
Externí odkaz:
https://doaj.org/article/a12e0df904a245719da86a701a141d78
Publikováno v:
Microbial Cell Factories, Vol 19, Iss 1, Pp 1-10 (2020)
Microbial Cell Factories
Microbial Cell Factories
Background Streptococcus thermophilus is an important food starter and receiving more attention to serve as cell factories for production of high-valued metabolites. However, the low yields of intracellular or extracellular expression of biotechnolog
Autor:
Hany Osman, Jennifer Eschbacher, Denah M. Appelt, Mark C. Preul, Steven S. Yocom, Joseph Georges, Mark Ishak, H. Warren Goldman, Brandon Boyer, Peter Nakaji, Robert Lynagh, Alan Turtz, Danielle Lopez, Michael Kakareka
Publikováno v:
Journal of Neurosurgery. 132:530-536
OBJECTIVEAccurate histopathological diagnoses are often necessary for treating neuro-oncology patients. However, stereotactic biopsy (STB), a common method for obtaining suspicious tissue from deep or eloquent brain regions, fails to yield diagnostic
Autor:
Hiroshi Takahashi, Yoichiro Masuda, Tsutomu Igarashi, Tadashi Nakano, Kotaro Oki, Maika Kobayashi
Publikováno v:
Journal of Cataract and Refractive Surgery. 45:1168-1171
Purpose To investigate the relationship between the generation of free radicals and femtosecond laser lens irradiation. Setting AMO Laboratory, Tokyo, Japan. Design Ex vivo studies. Methods Hydroxyphenyl fluorescein (HPF) was injected into the anteri
Autor:
Keiko Abe, Takanobu Sakurai, Naoki Mori, Akihito Yasuoka, Hirotatsu Kojima, Takumi Misaka, Takayoshi Okabe, Ryusei Matsuda, Hidenori Watanabe, Yoichi Kasahara, Tomiko Asakura, Masataka Narukawa
Publikováno v:
Bioscience, Biotechnology, and Biochemistry. 83:243-250
The epithelial sodium channel (ENaC) plays a pivotal role in sodium homeostasis, and the development of drugs that modulate ENaC activity is of great potential therapeutic relevance. We screened 6100 chemicals for their ability to activate sodium per
Publikováno v:
Journal of food scienceREFERENCES. 86(8)
HLE-B3 cell line, a human lens epithelial cell line, was used to examine the anti-glycative and anti-oxidative protection of aqueous extract prepared from steamed red amaranth leaves against high glucose induced injury. Phytochemical profile of this
Autor:
Mauricio Ramírez-Castrillón, Victoria P. Jaramillo-Garcia, Helio Lopes Barros, João A. Pegas Henriques, Valter Stefani, Patricia Valente
Publikováno v:
Frontiers in Microbiology
Frontiers in Microbiology, Vol 12 (2021)
Frontiers in Microbiology, Vol 12 (2021)
High-throughput screening methodologies to estimate lipid content in oleaginous yeasts use Nile red fluorescence in a given solvent and optimized excitation/emission wavelengths. However, Nile red fluorescence stabilization has been poorly analyzed,