Development of in vitro transposon assisted signal sequence trapping and its use in screening Bacillus halodurans C125 and Sulfolobus solfataricus P2 gene libraries

Autor: Jannick Dyrløv Bendtsen, Fiona Becker, Peter Bjarke Olsen, Kirk Matthew Schnorr, Reinhard Wilting, Niels Tolstrup

Publikováno v: Journal of Microbiological Methods. 57:123-133

To identify genes encoding extracytosolic proteins, a minitransposon, TnSig, containing a signal-less β-lactamase (′ bla ) as reporter gene, was constructed and used for in vitro transposition of genomic libraries made in Escherichia coli . The �

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::35a35bef8d8e87769479f98d97288507
https://doi.org/10.1016/j.mimet.2003.12.002

Identification and characterization of a bacterial glutamic peptidase

Autor: Reinhard Wilting, Kenneth Jensen, Søren Flensted Lassen, Peter Rahbek Østergaard

Publikováno v: BMC Biochemistry
Jensen, K, Østergaard, P R, Wilting, R & Lassen, S F 2010, ' Identification and characterization of a bacterial glutamic peptidase ', BMC Biochemistry, vol. 11, no. 47 . https://doi.org/10.1186/1471-2091-11-47
BMC Biochemistry, Vol 11, Iss 1, p 47 (2010)

Background Glutamic peptidases, from the MEROPS family G1, are a distinct group of peptidases characterized by a catalytic dyad consisting of a glutamate and a glutamine residue, optimal activity at acidic pH and insensitivity towards the microbial d

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c59c57b5ec372b384662c8127d3c3bbd
https://pubmed.ncbi.nlm.nih.gov/21122090