Zobrazeno 1 - 10
of 65
pro vyhledávání: '"Reinhard Guckenberger"'
Publikováno v:
Nature Nanotechnology. 2:220-225
The development of systematic approaches to explore protein–protein interactions and dynamic protein networks is at the forefront of biological sciences. Nanopatterned protein arrays offer significant advantages for sensing applications, including
Autor:
Reinhard Guckenberger, Nader A. Issa
Publikováno v:
Plasmonics. 2:31-37
Tapered metal wires show a remarkable ability to ‘squeeze’ the lateral extent of a propagating surface-plasmon-polariton mode as it travels toward the tip of the taper. The transformation can be continued well below the diffraction limit to termi
Publikováno v:
Review of Scientific Instruments. 75:2564-2568
We present an automated stabilization of the imaging process in tapping mode atomic force microscopy. For biological applications, the requirement of stable imaging conditions to achieve reliable high resolution is contradicted by the necessity to wo
Autor:
Andreas Thess, Wolfgang Baumeister, Robert Tampé, Matthias Hofmann, Silke Hutschenreiter, Reinhard Guckenberger
Publikováno v:
Journal of Biological Chemistry. 277:36321-36328
The potential of a protein-engineered His tag to immobilize macromolecules in a predictable orientation at metal-chelating lipid interfaces was investigated using recombinant 20 S proteasomes His-tagged in various positions. Electron micrographs demo
Publikováno v:
Ultramicroscopy. 90:103-112
A chemical procedure for anchoring DNA molecules to gold surfaces was used to facilitate the imaging of DNA and DNA–protein complexes in buffer solution by tapping mode atomic force microscopy (TMAFM). For preparing flat gold surfaces, a novel appr
Direct observation of different surface structures on high-resolution images of native halorhodopsin
Publikováno v:
Journal of Molecular Biology. 310:773-780
Halorhodopsin (HR) was investigated with atomic force microscopic techniques (AFM) in aqueous solution. Two-dimensional (2D) crystals of HR were obtained by purifying an HR membrane fraction with the same buoyant density as the purple membrane (HR-PM
Autor:
Matthias Amrein, Hans-Joachim Galla, A. Wintergalen, Reinhard Guckenberger, Manfred Sieber, A. Kramer
Publikováno v:
Biophysical Journal. 78(1):458-465
Lung surfactant films at the air/water interface exhibit the particularity that surfactant molecules are expelled from the surface monolayer into a surface associated multilamellar phase during compression. They are able to re-enter the surface film
Publikováno v:
Journal of Structural Biology. 127:113-119
Ribonucleic acids (RNAs) play a key role in many fundamental life processes. These polymers are often found complexed with proteins in extremely large particles whose molecular mass may reach several millions of daltons (e.g., ribosomes, spliceosomes
Publikováno v:
Nucleic Acids Research. 27:3645-3652
RNA polymerase was halted in consecutive registers of RNA synthesis ranging from registers 11 to 68. Non-denaturing gel electrophoresis shows that the mobility of the complexes varies (up to 15%), indicating that halted complexes differ in their conf
Publikováno v:
Molecular Crystals and Liquid Crystals Science and Technology. Section A. Molecular Crystals and Liquid Crystals. 329:171-178
Morphology of the liquid crystal/polymer composite has been investigated at the microscopic level as a model investigation in order to elucidate the nature of microscopic phase separation. It has b...