Relationships between Cargo, Cell Penetrating Peptides and Cell Type for Uptake of Non-Covalent Complexes into Live Cells

Autor: Andrea-Anneliese Keller, Franziska Mussbach, Reinhard Breitling, Peter Hemmerich, Buerk Schaefer, Stefan Lorkowski, Siegmund Reissmann

Publikováno v: Pharmaceuticals, Vol 6, Iss 2, Pp 184-203 (2013)

Modulating signaling pathways for research and therapy requires either suppression or expression of selected genes or internalization of proteins such as enzymes, antibodies, nucleotide binding proteins or substrates including nucleoside phosphates a

Externí odkaz: https://doaj.org/article/2ae3b329f7d14aaeae08cb0c1b969b7f

Transduction of Proteins intoLeishmania Tarentolaeby Formation of Non-Covalent Complexes With Cell-Penetrating Peptides

Autor: Peter Hemmerich, Stefan Lorkowski, Maria Braun, Andrea-Anneliese Keller, Katarina Kappe, Buerk Schaefer, Reinhard Breitling, Berith Wittig, Siegmund Reissmann

Publikováno v: Journal of Cellular Biochemistry. 115:243-252

Cell-penetrating peptides (CPPs) are used to transport peptides, proteins, different types of ribonucleic acids (or mimics of these molecules), and DNA into live cells, both plant and mammalian. Leishmania belongs to the class of protozoa having, in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e0a445e45d4fa401f650df81b2c7fc32
https://doi.org/10.1002/jcb.24654

Chemical and enzymatic characterization of recombinant rabbit muscle pyruvate kinase

Autor: Julia Linnemann, Reinhard Breitling, Siegmund Reissmann, Stefan Lorkowski, Christian Boehme, Frank Bieber

Publikováno v: bchm. 394:695-701

The stepwise synthesis of thymidine triphosphate (TTP) requires a kinase for phosphorylation in the last step. Because pyruvate kinase (PK) using phosphoenolpyruvate (PEP) as substrate can regenerate adenosine triphosphate and phosphorylate thymidine

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cc319d2b67958d8779900d54bcc541f
https://doi.org/10.1515/hsz-2012-0334

Insights on a putative aminoacyl-tRNA-protein transferase of Leishmania major

Autor: Felipe Freitas de Castro, Angela K. Cruz, Rohit Sharma, Reinhard Breitling, Eduardo B. Oliveira, Monica Terrao, Vitor M. Faça

Publikováno v: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
PLoS ONE, Vol 13, Iss 9, p e0203369 (2018)

The N-end rule pathway leads to regulated proteolysis as an adaptive response to external stress and is ubiquitous from bacteria to mammals. In this study, we investigated a gene coding for a putative core enzyme of this post-translational regulatory

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::17baa6acc07659ff9398483234310da2
https://doi.org/10.1371/journal.pone.0203369

Isotopic labeling of recombinant proteins expressed in the protozoan host Leishmania tarentolae

Autor: Mathias Gruen, Ion Cristian Cirstea, Reinhard Breitling, Tim Bergbrede, Kirill Alexandrov, Regina Pietrucha, Peter Bayer, Anca Niculae

Publikováno v: Protein Expression and Purification. 48:167-172

Isotope labeling of recombinant proteins is a prerequisite for application of nuclear magnetic resonance spectroscopy (NMR) for the characterization of the three-dimensional structures and dynamics of proteins. Overexpression of isotopically labeled

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7a59fb260a1300e3d494f55fed1c929
https://doi.org/10.1016/j.pep.2006.04.006