Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Reiko Onuki"'
Publikováno v:
Journal of Bioscience and Bioengineering. 134:348-355
Here we report the perfusion culture of a multi-layered tissue composed of HepG2 cells (a human hepatoma line) in a pressure-driven microphysiological system (PD-MPS), which we developed previously as a multi-throughput perfusion culture platform. Th
Autor:
Reiko Onuki-Nagasaki, Shinji Sugiura, Toshiyuki Kanamori, Tomoya Inoue, Taku Satoh, Hirotada Hirama, Kazumi Shin
Publikováno v:
Journal of Bioscience and Bioengineering. 127:641-646
The use of organ-on-a-chip (OOC) devices is a promising alternative to existing cell-based assays and animal testing in drug discovery. A rapid prototyping method with polydimethylsiloxane (PDMS) is widely used for developing OOC devices. However, be
Autor:
Reiko Onuki-Nagasaki1,2, Akira Nagasaki1 a-nagasaki@aist.go.jp, Kazumi Hakamada1,3,4, Uyeda, Taro Q. P.1, Masato Miyake1, Jun Miyake1,3, Satoshi Fujita1 s-fujita@aist.go.jp
Publikováno v:
BMC Genetics. 2015, Vol. 16 Issue 1, p1-12. 12p.
Autor:
Shinji Sugiura, Kazumi Shin, Toshiyuki Kanamori, Reiko Onuki-Nagasaki, S. Ishida, Taku Satoh, M. Kakiki, K. Kikuchi
Publikováno v:
Lab on a chip. 18(1)
This paper reports a multi-throughput multi-organ-on-a-chip system formed on a pneumatic pressure-driven medium circulation platform with a microplate-sized format as a novel type of microphysiological system. The pneumatic pressure-driven platform e
Publikováno v:
Sensors and Actuators B: Chemical. 190:896-899
A microdevice was designed to prevent cross-contamination of cells and reagents among reverse transfection (RTF) spots by using nanoliter-scale culture chambers. The forces generated by surface tension were used to seed cells into all the culture cha
Publikováno v:
Biofabrication. 9(1)
Traditional drug discovery involves the screening of lead compounds from a chemical library by using cell-based high throughput screening (HTS) procedures. This has created a demand for the development of cell-based microarray chips for HTS of compou
Publikováno v:
Experimental Cell Research. 313:3517-3525
Multidrug resistance-associated protein ABCC2 (MRP2) is widely expressed in mammalian tissues including intestine, liver and kidney, and it has been shown to be located exclusively on the apical membrane of polarized cells. Recently, several reports
Publikováno v:
Biochemical and Biophysical Research Communications. 360:615-620
Phosphorylated double-stranded RNA-dependent protein kinase (PKR) is thought to play an important role during ER stress induced cell death, but its molecular mechanism of action has not yet been clarified completely. To resolve this issue, we employe
Autor:
Hidetaka Akita, Sachiko Mita, Reiko Onuki, Alan F. Hofmann, Hisamitsu Hayashi, Yuichi Sugiyama, Hiroshi Suzuki
Publikováno v:
Drug Metabolism and Disposition. 34:1575-1581
Vectorial transport of bile acids across hepatocytes is a major driving force for bile flow, and bile acid retention in the liver causes hepatotoxicity. The basolateral and apical transporters for bile acids are thought to be targets of drugs that in
Autor:
Reiko Onuki, Hidetaka Akita, Hiroshi Suzuki, Sachiko Mita, Yuichi Sugiyama, Hisamitsu Hayashi, Alan F. Hofmann
Publikováno v:
American Journal of Physiology-Gastrointestinal and Liver Physiology. 290:G550-G556
Na+-taurocholate-cotransporting peptide (NTCP)/SLC10A1 and bile salt export pump (BSEP)/ABCB11 synergistically play an important role in the transport of bile salts by the hepatocyte. In this study, we transfected human NTCP and BSEP or rat Ntcp and