Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Reiko Kintaka"'
Autor:
Reiko Kintaka, Koji Makanae, Shotaro Namba, Hisaaki Kato, Keiji Kito, Shinsuke Ohnuki, Yoshikazu Ohya, Brenda J Andrews, Charles Boone, Hisao Moriya
Publikováno v:
eLife, Vol 9 (2020)
Overproduction (op) of proteins triggers cellular defects. One of the consequences of overproduction is the protein burden/cost, which is produced by an overloading of the protein synthesis process. However, the physiology of cells under a protein bu
Externí odkaz:
https://doaj.org/article/e6787edb0fe14313bbb3ee62e8ff839b
Publikováno v:
PLoS ONE, Vol 10, Iss 3, p e0120678 (2015)
In a previous study, we found an unknown element that caused growth inhibition after its copy number increased in the 3' region of DIE2 in Saccharomyces cerevisiae. In this study, we further identified this element and observed that overexpression of
Externí odkaz:
https://doaj.org/article/33302f2d4ebb49939be9145b860e29fa
Autor:
Reiko Kintaka, Hisao Moriya, Hisaaki Kato, Keiji Kito, Shinsuke Ohnuki, Charles Boone, Yoshikazu Ohya, Shotaro Namba, Koji Makanae, Brenda J. Andrews
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c9855f04e5412299997e54ca8309a47c
https://doi.org/10.7554/elife.54080.sa2
https://doi.org/10.7554/elife.54080.sa2
Autor:
Hisao Moriya, Reiko Kintaka, Yoshikazu Ohya, Shinsuke Ohnuki, Koji Makanae, Hisaaki Kato, Brenda J. Andrews, Charles Boone
Overproduction (op) of proteins triggers cellular defects. One of the defined consequences of protein overproduction is the protein burden/cost, which is produced by an overloading of the protein synthesis process. However, the physiology of cells un
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f79ccf32331d74e9abf29666aa703a97
Autor:
Reiko Kintaka, Yuichi Eguchi, Koji Makanae, Hisao Moriya, Shintaro Iwasaki, Yuichi Shichino, Nozomu Saeki, Nobutada Kimura, Manabu Kanno
Publikováno v:
Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Scientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
Extreme overproduction of gratuitous proteins can overload cellular protein production resources, leading to growth defects, a phenomenon known as the protein burden/cost effect. Genetic screening in the budding yeast Saccharomyces cerevisiae has iso
Autor:
Yoshikazu Ohya, Reiko Kintaka, Brenda J. Andrews, Shotaro Namba, Koji Makanae, Hisao Moriya, Charles Boone, Shinsuke Ohnuki, Hisaaki Kato, Keiji Kito
Publikováno v:
eLife
eLife, Vol 9 (2020)
eLife, Vol 9 (2020)
Overproduction (op) of proteins triggers cellular defects. One of the consequences of overproduction is the protein burden/cost, which is produced by an overloading of the protein synthesis process. However, the physiology of cells under a protein bu
Autor:
Yoichiro Ito, Akinori Ikeuchi, Mamoru Yamanishi, Chie Imamura, Satoshi Katahira, Reiko Kintaka, Takashi Matsuyama, Hisao Moriya
Publikováno v:
ACS Synthetic Biology. 2:337-347
The terminator regions of eukaryotes encode functional elements in the 3' untranslated region (3'-UTR) that influence the 3'-end processing of mRNA, mRNA stability, and translational efficiency, which can modulate protein production. However, the con
Publikováno v:
Scientific Reports
High-level expression of a protein localized to an intracellular compartment is expected to cause cellular defects because it overloads localization processes. However, overloads of localization processes have never been studied systematically. Here,
Evaluation of the lower protein limit in the budding yeast Saccharomyces cerevisiae using TIPI-gTOW.
Publikováno v:
BMC Systems Biology; 2014, Vol. 8 Issue 1, p1-22, 22p
Publikováno v:
BMC Systems Biology
Background Identifying permissible limits of intracellular parameters such as protein expression provides important information for examining robustness. In this study, we used the TEV protease-mediated induction of protein instability (TIPI) in comb