A fast and tuneable auxin-inducible degron for depletion of target proteins in budding yeast

Autor: Mendoza-Ochoa, Gonzalo I, Barrass, J David, Terlouw, Barbara R, Maudlin, Isabella E, de Lucas, Susana, Sani, Emanuela, Aslanzadeh, Vahid, Reid, Jane A E, Beggs, Jean D

Publikováno v: Mendoza-Ochoa, G I, Barrass, J D, Terlouw, B R, Maudlin, I E, de Lucas, S, Sani, E, Aslanzadeh, V, Reid, J A E & Beggs, J D 2019, ' A fast and tuneable auxin-inducible degron for depletion of target proteins in budding yeast ', Yeast, vol. 36, no. 1, pp. 75-81 . https://doi.org/10.1002/yea.3362

The auxin-inducible degron (AID) is a useful technique to rapidly deplete proteins of interest in non-plant eukaryotes. Depletion is achieved by addition of the plant hormone auxin to the cell culture, which allows the auxin-binding receptor, TIR1, t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=od______3094::feb1eb4b0bf055051da87e8b9698434f
https://www.pure.ed.ac.uk/ws/files/76666988/Mendoza_Ochoa_et_al_2018_Yeast.pdf