Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe

Autor: Tichaona Sagonda, Lucy Mupfumi, Rumbidzai Manzou, Beauty Makamure, Mqondisi Tshabalala, Lovemore Gwanzura, Peter Mason, Reggie Mutetwa

Publikováno v: Tuberculosis Research and Treatment, Vol 2014 (2014)

We conducted a cross-sectional study of second line drug resistance patterns and genetic diversity of MDR-TB isolates archived at the BRTI-TB Laboratory, Harare, between January 2007 and December 2011. DSTs were performed for second line antitubercul

Externí odkaz: https://doaj.org/article/8702ca7ec1f5400aa92b50e84b08c93e

Detection of Mycobacterium tuberculosis in sputum by gas chromatography-mass spectrometry of methyl mycocerosates released by thermochemolysis.

Autor: Denise M O'Sullivan, Simona C Nicoara, Reggie Mutetwa, Stanley Mungofa, Oona Y-C Lee, David E Minnikin, Max W Bardwell, Elizabeth L Corbett, Ruth McNerney, Geraint H Morgan

Publikováno v: PLoS ONE, Vol 7, Iss 3, p e32836 (2012)

Tuberculosis requires rapid diagnosis to prevent further transmission and allow prompt administration of treatment. Current methods for diagnosing pulmonary tuberculosis lack sensitivity are expensive or are extremely slow. The identification of lipi

Externí odkaz: https://doaj.org/article/1676daf77e1243729cffd1378802c9ab

Development of sample clean up methods for the analysis of Mycobacterium tuberculosis methyl mycocerosate biomarkers in sputum extracts by gas chromatography–mass spectrometry

Autor: Ruth McNerney, Liz E. Corbett, Reggie Mutetwa, Oona Y.-C. Lee, Nicholas W. Turner, Geraint Morgan, Denise M. O'Sullivan, David E. Minnikin, Simona Nicoara

Publikováno v: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

Highlights • We developed a sample clean-up method to detect tuberculosis from sputum by GC–MS. • Biomarkers recovered: 64–70% (standards solution), and 36–68% (sputum extracts). • Cholesterol removed: 93–98% (standards solution) and 62

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d4de2b622c0804cb65104b471b12392c
http://europepmc.org/articles/PMC4381843

Impact of Xpert MTB/RIF on Antiretroviral Therapy-Associated Tuberculosis and Mortality: A Pragmatic Randomized Controlled Trial

Autor: Lucy Mupfumi, Tichaona Sagonda, Beauty Makamure, John Z. Metcalfe, S. Zinyowera, Reggie Mutetwa, Peter R. Mason, Maxwell Chirehwa

Publikováno v: Open Forum Infectious Diseases

Introduction. GeneXpert® MTB/RIF (Xpert) is now widely distributed in high human immunodeficiency virus (HIV)/tuberculosis (TB)-burden countries. Yet, whether the test improves patient-important outcomes within HIV treatment programs in limited reso

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89ef42c0938f5fe47b6ccdae5204e29a
http://europepmc.org/articles/PMC4324195

Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of Drug-Resistant Tuberculosis in Harare, Zimbabwe

Autor: Salome Makumbirofa, Reggie Mutetwa, Jesca Mhaka, Lucy Mupfumi, John Z. Metcalfe, Peter R. Mason, Beauty Makamure

Publikováno v: PLoS ONE
PLoS ONE, Vol 8, Iss 2, p e55872 (2013)
Metcalfe, John; Makamure, B; Mhaka, J; Makumbirofa, S; Mutetwa, R; Mupfumi, L; et al.(2013). Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of Drug-Resistant Tuberculosis in Harare, Zimbabwe. UC San Francisco: Retrieved from: http://www.escholarship.org/uc/item/67b3b1h0

Introduction Limited data exist on use of the microscopic-observation drug-susceptibility (MODS) assay among persons suspected of MDR-TB living in high HIV-prevalence settings. Methods We retrospectively reviewed available clinical and drug susceptib

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e5aac57640ac0fd6b72da310f64e4d58
http://europepmc.org/articles/PMC3569460