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pro vyhledávání: '"Regan Szalay"'
Autor:
Jonathan Y. Hsu, Julian Grünewald, Regan Szalay, Justine Shih, Andrew V. Anzalone, Kin Chung Lam, Max W. Shen, Karl Petri, David R. Liu, J. Keith Joung, Luca Pinello
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-6 (2021)
Prime editing guide RNA design is more complex than for standard CRISPR-based nucleases or base editors. Here the authors present PrimeDesign and PrimeVar for the rapid and simplified design of pegRNA and ngRNA combinations.
Externí odkaz:
https://doaj.org/article/641f29fd5ac64f28bbea06a81c5212bd
Autor:
Luca Pinello, Max W. Shen, Regan Szalay, Julian Grünewald, Jonathan Y. Hsu, J. Keith Joung, Andrew V. Anzalone, Justine Shih, David R. Liu, Kin Chung Lam, Karl Petri
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-6 (2021)
Nature Communications
Nature Communications
Prime editing (PE) is a versatile genome editing technology, but design of the required guide RNAs is more complex than for standard CRISPR-based nucleases or base editors. Here we describe PrimeDesign, a user-friendly, end-to-end web application and