Zobrazeno 1 - 10
of 360
pro vyhledávání: '"Recombinase-mediated cassette exchange"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
Mammalian display enables the selection of biophysically favorable antibodies from a large IgG antibody library displayed on the plasma membrane of mammalian cells. We constructed and validated a novel mammalian display platform utilizing the commerc
Externí odkaz:
https://doaj.org/article/b1bb56f0fbea421aa58ac67ff7fc5d88
Autor:
Nina B. Reese, Sherman Ku
Publikováno v:
Current Research in Biotechnology, Vol 3, Iss , Pp 269-280 (2021)
Current methods for stably expressing recombinant protein therapeutics in CHO cells often rely on random or semi-random genomic integration events which result in a widely heterogeneous cell population. Consequently, a significant portion of cell lin
Externí odkaz:
https://doaj.org/article/2ff1ea56e0ab4582a3e41486760a50d4
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America, 2005 Aug . 102(35), 12483-12488.
Externí odkaz:
https://www.jstor.org/stable/3376604
Akademický článek
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Autor:
Aya Tanaka, Midori Yamana, Hisamitsu Ishihara, Suguru Yamaguchi, Akiko Nagasawa, Genta Kohno, Asami Furukawa, Minami Kosuda
Publikováno v:
Journal of Diabetes Investigation, Vol 12, Iss 12, Pp 2129-2140 (2021)
Journal of Diabetes Investigation
Journal of Diabetes Investigation
Aims/Introduction Recent studies have identified genomic and transcript level changes along with alterations in insulin secretion in patients with diabetes and in rodent models of diabetes. It is important to establish an efficient system for testing
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b616e7cf90d4389bf498e5cf043e46b4
https://doaj.org/article/8bbd79b70e0942e88c54cfef8c8c1d7a
https://doaj.org/article/8bbd79b70e0942e88c54cfef8c8c1d7a
Publikováno v:
Biotechnology and Bioprocess Engineering. 26:795-803
Traditional cell line development is based on random genomic integration of transgenes. Random integration leads to unpredictable expression and results in clonal heterogeneity requiring a tedious screening procedure. Therefore, a new strategy is nee
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6a1516a78a66b1ebbe68cb42a2a22d27
https://doi.org/10.1007/s12257-020-0332-y
https://doi.org/10.1007/s12257-020-0332-y
Publikováno v:
ACS Synthetic Biology. 10:1715-1727
A platform, based on targeted integration of transgenes using recombinase-mediated cassette exchange (RMCE) coupled with CRISPR/Cas9, is increasingly being used for the development of mammalian cell lines that produce therapeutic proteins, because of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8172ee3d8415cce74e79d9b8b73af981
https://doi.org/10.1021/acssynbio.1c00113
https://doi.org/10.1021/acssynbio.1c00113
Autor:
Ngan T B Nguyen, Mariati, Jessna H. M. Yeo, Terry Nguyen-Khuong, Jian'er Lin, Shi Jie Tay, Yuansheng Yang
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
Scientific Reports
Scientific Reports
Therapeutic antibodies are decorated with complex-type N-glycans that significantly affect their biodistribution and bioactivity. The N-glycan structures on antibodies are incompletely processed in wild-type CHO cells due to their limited glycosylati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d58d1a33f10e3ce4e3cf3fb1b06a7f96
https://doaj.org/article/d6016d0862bc4687baedcd7b078f5685
https://doaj.org/article/d6016d0862bc4687baedcd7b078f5685
Publikováno v:
Journal of Pharmaceutical Sciences. 110:946-951
Stable gene integration and rapid selection of high-expressing clones are important when developing biopharmaceutical systems to produce a protein of interest. According to regulatory guidelines, the final production clones should be stable through m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7c17a1c712f7e6e29fe6294aea800a0
https://doi.org/10.1016/j.xphs.2020.10.005
https://doi.org/10.1016/j.xphs.2020.10.005
Akademický článek
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