Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria

Autor: Fenryco Pratama, Neil Dixon, Dennis Linton

Publikováno v: Microbial Cell Factories
Microbial Cell Factories, Vol 20, Iss 1, Pp 1-25 (2021)
Pratama, F, Linton, D & Dixon, N 2021, ' Genetic and process engineering strategies for enhanced recombinant N-glycoprotein production in bacteria ', Microbial Cell Factories, vol. 20, no. 1, 198 . https://doi.org/10.1186/s12934-021-01689-x

Background The production of N-linked glycoproteins in genetically amenable bacterial hosts offers great potential for reduced cost, faster/simpler bioprocesses, greater customisation, and utility for distributed manufacturing of glycoconjugate vacci

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1242c77594226de11fc563c83bf9642b
http://europepmc.org/articles/PMC8518210

Liquid–Liquid Phase Separation Enhances TDP-43 LCD Aggregation but Delays Seeded Aggregation

Autor: Ludo Van Den Bosch, Frederic Rousseau, Emiel Michiels, Mathias De Decker, Peter Tompa, Sylvie Derclaye, Anna Bratek-Skicki, Philip Van Damme, David Alsteens, Joris Van Lindt, Donya Pakravan, Joost Schymkowitz

Publikováno v: Biomolecules
Volume 11
Issue 4
Biomolecules, Vol. 11, no.4, p. 548 (2021)
Biomolecules, Vol 11, Iss 548, p 548 (2021)

Aggregates of TAR DNA-binding protein (TDP-43) are a hallmark of several neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Although TDP-43 aggregates are an undisputed pathological species at the end stage of these diseases,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bd311aaacf6359f17dd6ba426b23cd54

Dysregulation of macrophage development and phenotype in diabetic human macrophages can be rescued by Hoxa3 protein transduction

Autor: Kimberly A. Mace, Edward A. McKenzie, Andrew J.M. Boulton, Tanja Torbica, Frank L. Bowling, Hadeel Al Sadoun, Salma Alrdahe

Publikováno v: Alrdahe, S, Al Sadoun, H, Torbica, T, McKenzie, E A, Bowling, F L, Boulton, A J M & Mace, K A 2019, ' Dysregulation of macrophage development and phenotype in diabetic human macrophages can be rescued by Hoxa3 protein transduction ', PLoS One., vol. 14, no. 10, pp. e0223980 . https://doi.org/10.1371/journal.pone.0223980, https://doi.org/10.1371/journal.pone.0223980
PLoS ONE, Vol 14, Iss 10, p e0223980 (2019)
PLoS ONE

Controlled inflammatory responses of myeloid cells recruited to wounds are essential for effective repair. In diabetes, the inflammatory response is prolonged and augmented over time, with increased myeloid cells present in the wound that fail to swi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d6ea39074a79895983e43418ef1be36
https://doi.org/10.1371/journal.pone.0223980

The three-dimensional structure of an H-superfamily conotoxin reveals a granulin fold arising from a common ICK cysteine framework

Autor: Anastasia Albert, Lars Ellgaard, Mads M. Foged, Terje Vasskog, Samuel D. Robinson, Cecilie L. Søltoft, Raymond S. Norton, Baldomero M. Olivera, Kaare Teilum, Andreas B. Bertelsen, Anthony W. Purcell, Lau Dalby Nielsen, Steen V. Petersen, Helena Safavi-Hemami

Publikováno v: Nielsen, L D, Foged, M M, Albert, A, Bertelsen, A B, Søltoft, C L, Robinson, S D, Petersen, S V, Purcell, A W, Olivera, B M, Norton, R S, Vasskog, T, Safavi-Hemami, H, Teilum, K & Ellgaard, L 2019, ' The three-dimensional structure of an H-superfamily conotoxin reveals a granulin fold arising from a common ICK cysteine framework ', The Journal of Biological Chemistry, vol. 294, no. 22, pp. 8745-8759 . https://doi.org/10.1074/jbc.RA119.007491
Nielsen, L D, Foged, M M, Albert, A, Bertelsen, A B, Søltoft, C L, Robinson, S D, Petersen, S V, Purcell, A W, Olivera, B M, Norton, R S, Vasskog, T, Safavi-Hemami, H, Teilum, K & Ellgaard, L 2019, ' The three-dimensional structure of an H-superfamily conotoxin reveals a granulin fold arising from a common ICK cysteine framework ', The Journal of biological chemistry . https://doi.org/10.1074/jbc.RA119.007491
J Biol Chem
Nielsen, L D, Foged, M M, Albert, A, Bertelsen, A B, Søltoft, C L, Robinson, S D, Petersen, S V, Purcell, A W, Olivera, B M, Norton, R S, Vasskog, T, Safavi-Hemami, H, Teilum, K & Ellgaard, L 2019, ' The three-dimensional structure of an H-superfamily conotoxin reveals a granulin fold arising from a common ICK cysteine framework ', Journal of Biological Chemistry, vol. 294, no. 22, pp. 8745-8759 . https://doi.org/10.1074/jbc.RA119.007491

Venomous marine cone snails produce peptide toxins (conotoxins) that bind ion channels and receptors with high specificity and therefore are important pharmacological tools. Conotoxins contain conserved cysteine residues that form disulfide bonds tha

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6471de4fea35e43d0dc539f45f90b2ee
https://curis.ku.dk/portal/da/publications/the-threedimensional-structure-of-an-hsuperfamily-conotoxin-reveals-a-granulin-fold-arising-from-a-common-ick-cysteine-framework(9a6f9a14-de9a-4f62-adfb-2ff58a676733).html

Kinetic analysis of PRMT1 reveals multifactorial processivity and a sequential ordered mechanism

Autor: Brown, Jennifer I, Koopmans, Timo, van Strien, Jolinde, Martin, Nathaniel I, Frankel, Adam, Afd Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery

Publikováno v: ChemBioChem, 19(1), 85-99
ChemBioChem
ChemBioChem, 19(1), 85. Wiley-VCH Verlag

Arginine methylation is a prevalent post‐translational modification in eukaryotic cells. Two significant debates exist within the field: do these enzymes dimethylate their substrates in a processive or distributive manner, and do these enzymes oper

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cafbfd3f390f40bbe48e99c46ebe50ac
http://hdl.handle.net/1887/69738