Clinical-grade whole genome sequencing-based haplarithmisis enables all forms of preimplantation genetic testing

Autor: Anouk E. J. Janssen, Rebekka M. Koeck, Rick Essers, Ping Cao, Wanwisa van Dijk, Marion Drüsedau, Jeroen Meekels, Burcu Yaldiz, Maartje van de Vorst, Bart de Koning, Debby M. E. I. Hellebrekers, Servi J. C. Stevens, Su Ming Sun, Malou Heijligers, Sonja A. de Munnik, Chris M. J. van Uum, Jelle Achten, Lars Hamers, Marjan Naghdi, Lisenka E. L. M. Vissers, Ron J. T. van Golde, Guido de Wert, Jos C. F. M. Dreesen, Christine de Die-Smulders, Edith Coonen, Han G. Brunner, Arthur van den Wijngaard, Aimee D. C. Paulussen, Masoud Zamani Esteki

Publikováno v: Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)

Abstract High-throughput sequencing technologies have increasingly led to discovery of disease-causing genetic variants, primarily in postnatal multi-cell DNA samples. However, applying these technologies to preimplantation genetic testing (PGT) in n

Externí odkaz: https://doaj.org/article/27a339f2ae754c6ab22930007a45ad79

At age 9, the methylome of assisted reproductive technology children that underwent embryo culture in different media is not significantly different on a genome-wide scale

Autor: Rebekka M Koeck, Florence Busato, Jorg Tost, Heleen Zandstra, Sylvie Remy, Sabine Langie, Marij Gielen, Ron van Golde, John C M Dumoulin, Han Brunner, Masoud Zamani Esteki, Aafke P A van Montfoort

Publikováno v: Human Reproduction, 37, 11, pp. 2709-2721
Human Reproduction, 37(11), 2709-2721. Oxford University Press
Human Reproduction, 37, 2709-2721

STUDY QUESTION Can we detect DNA methylation differences between ART children that underwent embryo culture in different media? SUMMARY ANSWER We identified no significant differences in site-specific or regional DNA methylation between the different

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2415386e141df26b824491e0a6715b9f
https://pubmed.ncbi.nlm.nih.gov/36206092