BioWF: A Naturally‐Fused, Di‐Domain Biocatalyst from Biotin Biosynthesis Displays an Unexpectedly Broad Substrate Scope

Autor: Shona M. Richardson, Peter J. Harrison, Michael A. Herrera, Menglu Wang, Rebecca Verez, Gustavo Perez Ortiz, Dominic J. Campopiano

Publikováno v: Richardson, S M, Harrison, P J, Herrera, M A, Wang, M, Verez, R, Ortiz, G P & Campopiano, D J 2022, ' BioWF: A naturally-fused, di-domain biocatalyst from biotin biosynthesis displays an unexpectedly broad substrate scope ', ChemBioChem . https://doi.org/10.1002/cbic.202200171

The carbon backbone of biotin is constructed from the C 7 di-acid pimelate, which is converted to an acyl-CoA thioester by an ATP-dependent, pimeloyl-CoA synthetase (PCAS, encoded by BioW). The acyl-thioester is condensed with ʟ-alanine in a decarbo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59aefe938aa9edcab1e582d114c15f2a
https://doi.org/10.1002/cbic.202200171

Enantiocomplementary Synthesis of γ-Nitroketones Using Designed and Evolved Carboligases

Autor: Xavier Garrabou, Donald Hilvert, Rebecca Verez

Publikováno v: Journal of the American Chemical Society. 139:103-106

Artificial enzymes created by computational design and directed evolution are versatile biocatalysts whose promiscuous activities represent potentially attractive starting points for divergent evolution in the laboratory. The artificial aldolase RA95

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13bb66689b5157183ba17788584923bb
https://doi.org/10.1021/jacs.6b11928