Multivalent interactions essential for lentiviral integrase function

Autor: Allison Ballandras-Colas, Vidya Chivukula, Dominika T. Gruszka, Zelin Shan, Parmit K. Singh, Valerie E. Pye, Rebecca K. McLean, Gregory J. Bedwell, Wen Li, Andrea Nans, Nicola J. Cook, Hind J. Fadel, Eric M. Poeschla, David J. Griffiths, Javier Vargas, Ian A. Taylor, Dmitry Lyumkis, Hasan Yardimci, Alan N. Engelman, Peter Cherepanov

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-16 (2022)

The authors determined high-resolution cryo-EM structures of the lentiviral intasome — the nucleoprotein complex that inserts viral DNA into a host chromosome — and show that the architecture comprising 16 integrase subunits is critical for its f

Externí odkaz: https://doaj.org/article/38b768c566d24b6c8e369c087cb41667

A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses

Autor: Tiong Kit Tan, Pramila Rijal, Rolle Rahikainen, Anthony H. Keeble, Lisa Schimanski, Saira Hussain, Ruth Harvey, Jack W. P. Hayes, Jane C. Edwards, Rebecca K. McLean, Veronica Martini, Miriam Pedrera, Nazia Thakur, Carina Conceicao, Isabelle Dietrich, Holly Shelton, Anna Ludi, Ginette Wilsden, Clare Browning, Adrian K. Zagrajek, Dagmara Bialy, Sushant Bhat, Phoebe Stevenson-Leggett, Philippa Hollinghurst, Matthew Tully, Katy Moffat, Chris Chiu, Ryan Waters, Ashley Gray, Mehreen Azhar, Valerie Mioulet, Joseph Newman, Amin S. Asfor, Alison Burman, Sylvia Crossley, John A. Hammond, Elma Tchilian, Bryan Charleston, Dalan Bailey, Tobias J. Tuthill, Simon P. Graham, Helen M. E. Duyvesteyn, Tomas Malinauskas, Jiandong Huo, Julia A. Tree, Karen R. Buttigieg, Raymond J. Owens, Miles W. Carroll, Rodney S. Daniels, John W. McCauley, David I. Stuart, Kuan-Ying A. Huang, Mark Howarth, Alain R. Townsend

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)

Vaccines for SARS-COV-2 are needed in the ongoing pandemic. Here the authors characterize a vaccine candidate that presents the receptor-binding domain (RBD) of SARS-CoV-2 spike protein on a synthetic VLP platform using SpyTag/SpyCatcher technology a

Externí odkaz: https://doaj.org/article/6b95639b85804b348dfe9af334fe3bcf

Vaccine Development for Nipah Virus Infection in Pigs

Autor: Rebecca K. McLean, Simon P. Graham

Publikováno v: Frontiers in Veterinary Science, Vol 6 (2019)

Nipah virus (NiV) causes a severe and often fatal neurological disease in humans. Whilst fruit bats are considered the natural reservoir, NiV also infects pigs and may cause an unapparent or mild disease. Direct pig-to-human transmission was responsi

Externí odkaz: https://doaj.org/article/ea2a3301137b4fd683d22e9520d46475

A COVID-19 vaccine candidate using SpyCatcher multimerization of the SARS-CoV-2 spike protein receptor-binding domain induces potent neutralising antibody responses

Autor: Julia A. Tree, Tomas Malinauskas, Mark Howarth, Adrian K. Zagrajek, Veronica Martini, John W. McCauley, Ryan Waters, Jiandong Huo, Jane C. Edwards, Ginette Wilsden, Holly Shelton, Alison Burman, Anna B. Ludi, Rolle Rahikainen, Rod S. Daniels, Isabelle Dietrich, Huang K-Ya., David I. Stuart, Simon P. Graham, M. Pedrera, Valerie Mioulet, Raymond J. Owens, Christopher Chiu, Pramila Rijal, Nazia Thakur, Philippa Hollinghurst, Sushant Bhat, Hayes Jwp., Matthew Tully, Alain Townsend, Miles W. Carroll, Saira Hussain, Clare Browning, Tobias J. Tuthill, Tiong Kit Tan, Katy Moffat, Dagmara Bialy, Rebecca K. McLean, Elma Tchilian, Karen R. Buttigieg, Sylvia Crossley, Ashley R. Gray, Carina Conceicao, Joseph Newman, Phoebe Stevenson-Leggett, Ruth Harvey, Bryan Charleston, Mehreen Azhar, Dalan Bailey, Amin S. Asfor, Duyvesteyn Hme., Anthony H. Keeble, John A. Hammond, Lisa Schimanski

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Nature Communications

There is need for effective and affordable vaccines against SARS-CoV-2 to tackle the ongoing pandemic. In this study, we describe a protein nanoparticle vaccine against SARS-CoV-2. The vaccine is based on the display of coronavirus spike glycoprotein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1a8ab4dac73be5c18bbdb35830cab842
https://doaj.org/article/6b95639b85804b348dfe9af334fe3bcf

Prevalence and Epidemiology of Non-O157 Escherichia coli Serogroups O26, O103, O111, and O145 and Shiga Toxin Gene Carriage in Scottish Cattle, 2014–2015

Autor: David L. Gally, Rebecca K McLean, Margo Chase-Topping, George J. Gunn, Helen Williamson, Marianne Keith, Carol G. Currie, Ian Handel, Nuno Silva, Lesley Allison, Heather A. Mathie, Thibaud Porphyre, MK Henry, Anne Holmes, J Evans, Sue C. Tongue, Rebecca Callaby, Deborah V. Hoyle, Kareen Macleod

Publikováno v: Hoyle, D, Keith, M, Williamson, H, Macleod, K, Mathie, H, Handel, I, Currie, C, Holmes, A, Allison, L, McLean, B, Callaby, R, Porphyre, T, Tongue, S C, Henry, M K, Evans, J, Gunn, G J, Gally, D, Silva, N A & Chase-Topping, M 2021, ' Prevalence and epidemiology of non-O157 Escherichia coli serogroups O26, O103, O111, O145 and Shiga toxin gene carriage in Scottish cattle, 2014-2015. ', Applied and Environmental Microbiology . https://doi.org/10.1128/AEM.03142-20
Applied and Environmental Microbiology
Applied and Environmental Microbiology, 2021, 87 (10), ⟨10.1128/AEM.03142-20⟩

Cattle are reservoirs for Shiga toxin Escherichia coli (STEC), bacteria shed in animal feces. Humans are infected through consumption of contaminated food or water and by direct contact, causing serious disease and kidney failure in the most vulnerab

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c06c0979b0c0687a8fb65e1901ef49e1
https://doi.org/10.1128/aem.03142-20