Zobrazeno 1 - 10
of 3 310
pro vyhledávání: '"Real-time quantitative PCR"'
Autor:
Saiyi Sun, Binglei Zhang, Wanhang Jia, Jiaxin Yang, Saiqiao Wang, Lu Zhao, Yan Ma, Qiujue Wu, Yuqin Wang
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-16 (2024)
Abstract Our study concentrated on the expression of miRNA-375 in the hypothalamic-pituitary-gonadal axis of female Hu sheep. The investigation involved cloning the precursor sequence of miR-NA-375, followed by comparison with database entries and su
Externí odkaz:
https://doaj.org/article/bf14e994b5ce406c92e3d6cdf6cd5332
Autor:
Aapo Juutinen, Ananda Tiwari, Anna-Maria Hokajärvi, Oskari Luomala, Aleksi Kolehmainen, Eveliina Nurmi, Elisa Salmivirta, Tarja Pitkänen, Anssi Lipponen
Publikováno v:
Scientific Reports, Vol 14, Iss 1, Pp 1-9 (2024)
Abstract Reverse transcription-quantitative PCR (RT-qPCR) is widely used for monitoring viruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in wastewater. Various materials, including plasmid DNA, synthetic nucleic acids,
Externí odkaz:
https://doaj.org/article/19142f7ee9e84cf690e45f4da2230525
Publikováno v:
Current Issues in Molecular Biology, Vol 46, Iss 10, Pp 10779-10794 (2024)
Paper Mulberry (Broussonetia papyrifera) possesses medicinal, economic, and ecological significance and is extensively used for feed production, papermaking, and ecological restoration due to its ease of propagation, rapid growth rate, and strong str
Externí odkaz:
https://doaj.org/article/f27dc24fdc5449b89fc420669b4b52df
Publikováno v:
Guangdong nongye kexue, Vol 51, Iss 8, Pp 21-30 (2024)
【Objective】Real-time quantitative PCR is one of the most conventional and efficient techniques for studying the mRNA expression level of target genes. The proper selection of reference genes is of great significance for the accuracy of the result
Externí odkaz:
https://doaj.org/article/3401428fb6034d43a46761a3a1fce03c
Publikováno v:
Current Issues in Molecular Biology, Vol 46, Iss 9, Pp 9449-9462 (2024)
Liquidambar formosana Hance is renowned for its rich leaf color and possesses notable advantages, such as robust adaptability, strong resistance to diseases and pests, and rapid growth, making it a preferred choice for urban greening and carbon seque
Externí odkaz:
https://doaj.org/article/92aea5f36f124b9f9e429cf80b99ab4f
Publikováno v:
Veterinární Medicína, Vol 69, Iss 7, Pp 234-242 (2024)
Peste des petits ruminants (PPR) is a serious acute, highly contagious disease caused by the peste des petits ruminants virus (PPRV). This study aims to establish a qRT-PCR assay with an internal amplification control for the rapid and accurate detec
Externí odkaz:
https://doaj.org/article/d0a32ad467494e4e978915c5ae02d369
Autor:
Luzheng Liu, Jiacheng Chen, Fei Ye, Fengran Chu, Chaoluan Rao, Yong Wang, Yanggang Yan, Jincai Wu
Publikováno v:
Discover Oncology, Vol 15, Iss 1, Pp 1-18 (2024)
Abstract Purpose Hepatocellular carcinoma (HCC) is the most prevalent malignancies worldwide. Recently, oxidative phosphorylation (OXPHOS) has received extensive concern as an emerging target in antitumor therapy. However, the OXPHOS-involved underly
Externí odkaz:
https://doaj.org/article/c8b2150993aa4c5ca012421c5b1b68b5
Publikováno v:
All Life, Vol 17, Iss 1 (2024)
Determination of the genomic copy number of Cre transgene is critical for the establishment of transgenic animal models. We demonstrate a real-time quantitative polymerase chain reaction (RT-qPCR) based method for genomic copy number determination of
Externí odkaz:
https://doaj.org/article/60dccc766b2d42349721418f13dcbb07
Autor:
Likun Long, Ning Zhao, Congcong Li, Yuxuan He, Liming Dong, Wei Yan, Zhenjuan Xing, Wei Xia, Yue Ma, Yanbo Xie, Na Liu, Feiwu Li
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
As one of the developed genetically modified (GM) maize varieties in China, CC-2 has demonstrated promising commercial prospects during demonstration planting. The establishment of detection methods is a technical prerequisite for effective supervisi
Externí odkaz:
https://doaj.org/article/994d187476e8471280880ada46eacf18