Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Raymonde Girardot"'
Publikováno v:
Infection and Immunity. 77:266-273
In this study, the binding of F components of the staphylococcal bicomponent leukotoxins Panton-Valentine leucocidin (LukF-PV) and gamma-hemolysin (HlgB) on polymorphonuclear neutrophils (PMNs), monocytes, and lymphocytes was determined using labeled
Publikováno v:
Current Microbiology. 26:53-56
We have isolated and purified enolase fromClostridium difficile. This is the first report of an enolase of theClostridium genus, and in general its characteristics resemble those described previously for other species, except that it is extremely the
Publikováno v:
Toxicon. 30:129-140
We compared the immunological properties and cytotoxic effects of Clostridium difficile toxin B and Clostridium sordellii toxin L. These two cytotoxins are immunologically related in that the cytotoxic effect of either toxin can be neutralized by the
Publikováno v:
Journal of Medical Microbiology. 31:251-257
Summary. Antisera against Clostridium difficile toxin B were prepared in sheep and rabbit and were used in indirect and sandwich enzyme-linked immunosorbent assays (ELISA) for the detection of toxin B. Polyvinyl chloride and polystyrene microtitratio
Publikováno v:
Journal of medical microbiology. 44(1)
In ileal loop assay, ELISA and anion-exchange column chromatography, Clostridium sordellii strain 6018 was shown to produce a cytotoxin, but no detectable enterotoxin. DNA sequence and polymerase chain reaction analyses indicated that the lack of ent
Publikováno v:
Toxicon : official journal of the International Society on Toxinology. 32(12)
The cytotoxins from Clostridium difficile (toxin B) and Clostridium sordellii (toxin L) induce rounding of cultured cells. The cellular effects induced by these two cytotoxins are clearly distinct, suggesting that both toxins use a similar, but not i
Publikováno v:
Biochemical and biophysical research communications. 203(1)
Exposure of McCoy cultured cells to Clostridium difficile cytotoxin B or okadaic acid (OA), a potent phosphatase inhibitor, results in similar morphological changes. Using two-dimensional gel electrophoresis, we have detected a protein of approximate
Publikováno v:
Natural toxins. 1(6)
Clostridium difficile toxin B and Clostridium sordellii toxin L, which are immunologically related toxins, possess a cytotoxic activity inducing depolymerization of microfilaments and cellular retraction of cell bodies that are different for toxin B-
Publikováno v:
Journal of chromatography. 528(2)
We have developed a rapid method for the purification of proteins, combining titration curve analysis with a two-step column chromatographic procedure. We have used this approach to purify the cytotoxin (L toxin) from Clostridium sordellii . We have
Publikováno v:
Biology of the Cell. 53:23-32
Clostridium difficile is the major etiologic agent of human pseudomembranous colitis. It produces two toxins: an enterotoxin and a cytotoxin. In cultured hepatoma cells, at very low doses, the cytotoxin inhibits the incorporation of precursors into b