Zobrazeno 1 - 4
of 4
pro vyhledávání: '"Raymond Shiaw-Ching Fong"'
Publikováno v:
Journal of Molecular Biology. 240:119-126
A detailed kinetic analysis demonstrates that, in vitro , mutations in the P RM promoter of bacteriophage λ can increase the rate of open complex formation at the divergent, lytic promoter P R in either of two ways. (1) P - RM mutations, typified by
Publikováno v:
Journal of Molecular Biology. 229:37-51
We have deleted a single base-pair in the -35 region of the bacteriophage lambda PRM promoter. The deletion (PRM delta 34) creates a better match of PRM to consensus, thereby substantially increasing the activity of the promoter in vitro and in vivo.
Publikováno v:
Journal of bacteriology. 175(17)
A cryptic promoter, designated P alpha, initiates transcription within the O(R) region of bacteriophage lambda. Transcription from P alpha proceeds in the direction of the cI repressor gene from sites 46 and 48 bp preceding the PRM transcription star
Modulation of P(RM) activity by the lambda PR promoter in both the presence and absence of repressor
Publikováno v:
Journal of molecular biology. 232(3)
When the transcription startsites of the phage lambda promoters PRM and PR are separated by 82 bp (the wild-type spacing), mutating PR increases the rate of open complex formation at PRM at all RNA polymerase (RNAP) concentrations tested in vitro. Th