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pro vyhledávání: '"Raymond Kunikane, Terhune"'
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 2312
Developments in genome-editing technology, especially CRISPR-Cas9, have revolutionized the way in which genetically engineered animals are generated. However, the process of generation includes microinjection to the one-cell stage embryo and the tran
Publikováno v:
Methods in Molecular Biology ISBN: 9781071614402
Developments in genome-editing technology, especially CRISPR-Cas9, have revolutionized the way in which genetically engineered animals are generated. However, the process of generation includes microinjection to the one-cell stage embryo and the tran
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::894a2ce3664016011cb0ba377acff399
https://doi.org/10.1007/978-1-0716-1441-9_19
https://doi.org/10.1007/978-1-0716-1441-9_19
Autor:
Yuji Tsunekawa, Taeko Suetsugu, Fumio Matsuzaki, Atsunori Shitamukai, Raymond Kunikane Terhune, Ikumi Fujita
Publikováno v:
Development (Cambridge, England)
Genome-editing technology has revolutionized the field of biology. Here, we report a novel de novo gene-targeting method mediated by in utero electroporation into the developing mammalian brain. Electroporation of donor DNA with the CRISPR/Cas9 syste