Zobrazeno 1 - 10
of 62
pro vyhledávání: '"Raymond F. Chen"'
Publikováno v:
Biochemistry. 30:5184-5195
The multiexponential decay of tryptophan derivatives has previously been explained by the presence of rotamers having different fluorescence lifetimes, but it has been difficult to correlate rotamer structure and physical properties. New time-resolve
Externí odkaz:
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https://doi.org/10.1021/bi00235a011
https://doi.org/10.1021/bi00235a011
Autor:
Jay R. Knutson, Myun Ki Han, Preston Hensley, Denise Porter, Soon-Jong Kim, Samuel H. Wilson, Cynthia K. Williamson, Raymond F. Chen, Michael J. Clague
Publikováno v:
SPIE Proceedings.
The authors outline some examples of the advantages found in subdividing overall quenching into heterogeneous contributions. Subdivision is accomplished by overdetermination (global) and association (DAS, decay associated spectral) methods. In some c
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https://doi.org/10.1117/12.58205
https://doi.org/10.1117/12.58205
Autor:
Raymond F. Chen
Publikováno v:
Analytical Letters. 7:65-77
Fluorescamine reacts rapidly with proteins to produce conjugates having A light blue fluorescence. The quantum yields of various conjugates were found to differ significantly, and the measured lifetimes at 25° ranged from 6 to 9 nsec. The yields wer
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https://doi.org/10.1080/00032717408059019
https://doi.org/10.1080/00032717408059019
Autor:
Raymond F. Chen
Publikováno v:
Analytical Letters. 11:249-255
The rate of reaction of fluorescamine with proline is faster than with primary amino acids. Proline competitively inhibits the fluorogenic reaction of primary amines with fluorescamine, the degree of inhibition being proportional to the amount of pro
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https://doi.org/10.1080/00032717808059698
https://doi.org/10.1080/00032717808059698
Publikováno v:
Analytical Letters. 21:2017-2032
Lytic peptides such as melittin and mastoparan are usually assayed by measuring the leakage of cell contents; e.g., hemolysis. When such peptides lyse liposomes containing concentration-quenched 6-carboxyfluorescein (6CF), the resulting fluorescence
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https://doi.org/10.1080/00032718808059888
https://doi.org/10.1080/00032718808059888
Autor:
Raymond F. Chen, Carrie H. Scott
Publikováno v:
Analytical Letters. 18:393-421
Fluorescence spectra and lifetimes of ovalbumin labeled with a variety of fluorescent reagents have been measured. The peak positions of the emission spectrum and the long wavelength band of the excitation spectrum as well as the fluorescence lifetim
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https://doi.org/10.1080/00032718508066142
https://doi.org/10.1080/00032718508066142
Autor:
Raymond F. Chen
Publikováno v:
Analytical Letters. 14:1591-1601
Texts on fluorescence spectroscopy state that the observed intensity of fluorescence is inversely proportional to the square of the refractive index of the solvent. This statement is evaluated for various arrangements of slits-To test the effect of r
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ccfe7c8a23327c519d4e27311282f100
https://doi.org/10.1080/00032718108059823
https://doi.org/10.1080/00032718108059823
Publikováno v:
Journal of Biological Chemistry. 249:2285-2293
The complementation of fragments of staphylococcal nuclease to form an ordered structure was studied by stopped flow kinetic measurements. The increase in the intensity of tryptophan fluorescence of residue 140 on complementation was followed. The co
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https://doi.org/10.1016/s0021-9258(19)42830-5
https://doi.org/10.1016/s0021-9258(19)42830-5
Autor:
Raymond F. Chen
Publikováno v:
Analytical Letters. 17:1225-1243
The use of fluorescence difference decay curves was explored as a way to isolate the decays of components in a complex system. Time-correlated single photon methodology allows one to subtract a “reference” curve from a “sample” curve from a
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https://doi.org/10.1080/00032718408065301
https://doi.org/10.1080/00032718408065301