Two beta-alanyl-CoA:ammonia lyases in Clostridium propionicum

Autor: Steve J. Gort, Gloria Herrmann, Olga V. Selifonova, Thorsten Selmer, Holly J. Jessen, Ravi R. Gokarn, Wolfgang Buckel

Publikováno v: FEBS Journal. 272:813-821

The fermentation of β-alanine by Clostridium propionicum proceeds via activation to the CoA-thiol ester, followed by deamination to acryloyl-CoA, which is also an intermediate in the fermentation of l-alanine. By shifting the organism from the carbo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::0428012d6a76251c0eb96d546ed592e7
https://doi.org/10.1111/j.1742-4658.2004.04518.x

The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli

Autor: Scott A. Martin, Mark A. Eiteman, Ravi R. Gokarn, Jennifer R. Walker, J D Evans, Elliot Altman

Publikováno v: Applied Microbiology and Biotechnology. 56:188-195

Oxaloacetate (OAA) plays an important role in the tricarboxylic acid cycle and for the biosynthesis of a variety of cellular compounds. Some microorganisms, such as Rhizobium etli and Corynebacterium glutamicum, are able to synthesize OAA during grow

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e96562e9106dc5342bc2d90db7810221
https://doi.org/10.1007/s002530100661

Discovery of enzymatic activity using stable isotope metabolite labeling and liquid chromatography-mass spectrometry

Autor: Hans H. Liao, Joseph J. Dalluge, Ravi R. Gokarn, Holly J. Jessen

Publikováno v: Analytical chemistry. 77(20)

Stable isotope labeling of an intracellular chemical precursor or metabolite allows direct detection of downstream metabolites of that precursor, arising from novel enzymatic activity of interest, using metabolite profiling liquid chromatography-mass

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b44f518d84b97ece437272dc249de28
https://pubmed.ncbi.nlm.nih.gov/16223264

Metabolic analysis of Escherichia coli in the presence and absence of the carboxylating enzymes phosphoenolpyruvate carboxylase and pyruvate carboxylase

Autor: Elliot Altman, Mark A. Eiteman, Ravi R. Gokarn

Publikováno v: Applied and environmental microbiology. 66(5)

Fermentation patterns of Escherichia coli with and without the phosphoenolpyruvate carboxylase (PPC) and pyruvate carboxylase (PYC) enzymes were compared under anaerobic conditions with glucose as a carbon source. Time profiles of glucose and ferment

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0befa3f7fcf37e3e93ed7b12cbbdbb34
https://pubmed.ncbi.nlm.nih.gov/10788348

Discovery of enzymatic activity using stable isotope metabolite labeling and liquid chromatography-mass spectrometry.

Autor: Dalluge JJ; Cargill Scientific Resources Center and Biotechnology Development Center, Cargill Incorporated, P.O. 5702, Minneapolis, Minnesota 55440-5702, USA. joseph_dalluge@cargill.com, Liao H, Gokarn R, Jessen H

Publikováno v: Analytical chemistry [Anal Chem] 2005 Oct 15; Vol. 77 (20), pp. 6737-40.