Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Ravi Kambampati"'
Autor:
Susan Peng, Nancy M. Dahms, Ravi Kambampati, Xu Wang, Jonathan H. LeBowitz, Peggy Tom, John Maga, Richard N. Bohnsack, Jianghong Zhou, Angela M. Thomm, Barry J. Byrne, Sarah Golata
Publikováno v:
The Journal of Biological Chemistry
Background: Acid α-glucosidase, an enzyme replacement therapy for Pompe disease, is poorly targeted to lysosomes when relying on phosphomannose residues. Results: Fusing IGF-II to acid α-glucosidase resulted in more efficient uptake and glycogen cl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05d0e09aeec5bd4ae887d48d36e2e357
https://doi.org/10.1074/jbc.m112.438663
https://doi.org/10.1074/jbc.m112.438663
Publikováno v:
Cornea. 26:1213-1219
PURPOSE To determine matrix metalloproteinase (MMP) 2 and MMP 9 expression in acute and chronic experimentally wounded canine corneas and keratectomy samples from canine patients with spontaneous chronic corneal epithelial defects (SCCEDs). METHODS M
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d03898f33bc14f79ab8bae765027801
https://doi.org/10.1097/ico.0b013e31814b8a28
https://doi.org/10.1097/ico.0b013e31814b8a28
Autor:
Ravi Kambampati, Charles T. Lauhon
Publikováno v:
Biochemistry. 42:1109-1117
Thionucleosides are uniquely present in tRNA. In many organisms, tRNA specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s2U) derivatives at wobble position 34. The s2 group of s2U34 stabilizes anticodon structure, confers ribosome b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::23aa93cf2e65c2c229e38f2c45f4e1a4
https://doi.org/10.1021/bi026536+
https://doi.org/10.1021/bi026536+
Autor:
Anthony A. Paiva, Lee Huang, Liane M. Mende-Mueller, Ravi Kambampati, Carla B. Pellegrino, Kalpana Chakraburtty
Publikováno v:
Journal of Biological Chemistry. 275:16963-16968
Elongation factor 3 (EF-3) is an ATPase essential for polypeptide chain synthesis in a variety of yeasts and fungi. We used limited proteolysis to study the organization of the subdomains of EF-3. Trypsinolysis of EF-3 at 30 °C resulted in the forma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3b545b71e2c96cb333f953976b144e77
https://doi.org/10.1074/jbc.m001157200
https://doi.org/10.1074/jbc.m001157200
Autor:
Ravi Kambampati, Charles T. Lauhon
Publikováno v:
Journal of Biological Chemistry. 275:10727-10730
IscS from Escherichia coli is a cysteine desulfurase that has been shown to be involved in Fe-S cluster formation. The enzyme converts L-cysteine to L-alanine and sulfane sulfur (S(0)) in the form of a cysteine persulfide in its active site. Recently
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3153ba76b85e4b3945c7571b51fda974
https://doi.org/10.1074/jbc.275.15.10727
https://doi.org/10.1074/jbc.275.15.10727
Autor:
Ravi Kambampati, Charles T. Lauhon
Publikováno v:
Biochemistry. 38:16561-16568
We have improved the in vitro assay for 4-thiouridine (s(4)U) biosynthesis in Escherichia coli tRNA by substituting an unmodified tRNA transcript as substrate and including recombinant ThiI protein, a known factor required for s(4)U synthesis. Using
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30915df9733e6c958a2dcac4f9219de8
https://doi.org/10.1021/bi991119r
https://doi.org/10.1021/bi991119r
Publikováno v:
European Journal of Biochemistry. 258:986-993
Elongation factor 3 (EF-3) is an essential requirement for translation in fungi. We previously reported activation of EF-3-ATPase by yeast ribosomes. EF-3 interacts with both ribosomal subunits and shows high affinity for 60S subparticles. Translatio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::38614cec63c6b2fb55fe05a87cf5c250
https://doi.org/10.1046/j.1432-1327.1998.2580986.x
https://doi.org/10.1046/j.1432-1327.1998.2580986.x
Autor:
Ravi Kambampati, Kalpana Chakraburtty
Publikováno v:
Journal of Biological Chemistry. 272:6377-6381
Elongation factor 3 (EF-3) is an essential requirement of the fungi for translational elongation. EF-3 is an ATPase, and the hydrolytic activity is stimulated 2 orders of magnitude by yeast ribosomes. Limited trypsinolysis of EF-3 results in the clea
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d8cdad4baaa86ac8f5a187d41bb0b28d
https://doi.org/10.1074/jbc.272.10.6377
https://doi.org/10.1074/jbc.272.10.6377
Autor:
Ravi, Kambampati, Charles T, Lauhon
Publikováno v:
Biochemistry. 42(4)
Thionucleosides are uniquely present in tRNA. In many organisms, tRNA specific for Lys, Glu, and Gln contain hypermodified 2-thiouridine (s(2)U) derivatives at wobble position 34. The s(2) group of s(2)U34 stabilizes anticodon structure, confers ribo
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7d25fd0d07730bd2d3815b1cd97c3551
https://pubmed.ncbi.nlm.nih.gov/12549933
https://pubmed.ncbi.nlm.nih.gov/12549933
Autor:
Ravi Kambampati, Kalpana Chakraburtty
Publikováno v:
Protein expression and purification. 10(2)
The translational elongation factor 3 (EF-3) from Saccharomyces cerevisiae was overexpressed and purified to near homogeneity from the post-ribosomal supernatant fraction (S-100). A detailed protocol for the isolation of overexpressed EF-3 is present
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9df76a290bbc06220f34049749b376c4
https://pubmed.ncbi.nlm.nih.gov/9226717
https://pubmed.ncbi.nlm.nih.gov/9226717