Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Rasmus Ø. Thorsen"'
Autor:
Christiaan N. Hulleman, Rasmus Ø. Thorsen, Eugene Kim, Cees Dekker, Sjoerd Stallinga, Bernd Rieger
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Molecular orientation is often ignored during single-molecule localisation microscopy. Here, the authors use a Vortex point spread function in order to simultaneously estimate the 3D position, dipole orientation and degree of rotational constraint, w
Externí odkaz:
https://doaj.org/article/2eb59b82dd434b909cb69b611e4e389f
Autor:
Rasmus Ø. Thorsen, Samel Arslanagić
Publikováno v:
Photonics, Vol 2, Iss 3, Pp 773-794 (2015)
The present work shows how the eccentricity in active nano-particles may lead to very interesting and rather directive near- and far-field radiation patterns. The nano-particle is of a three-layer type and consists of a silica core, a free-space midd
Externí odkaz:
https://doaj.org/article/ba3e93aa21d5432eb3a920a233b32558
Publikováno v:
Biomed Opt Express
Biomedical Optical Express, 13(5)
Biomedical Optical Express, 13(5)
Combining orientation estimation with localization microscopy opens up the possibility to analyze the underlying orientation of biomolecules on the nanometer scale. Inspired by the recent improvement of the localization precision by shifting excitati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af985345fde4ed47a689de008162ba55
https://doi.org/10.1364/boe.452159
https://doi.org/10.1364/boe.452159
We have developed an engineered Point Spread Function (PSF) to enable the simultaneous estimation of dipole orientation, 3D position and degree of rotational constraint of single-molecule emitters from a single 2D focal plane. Besides giving access t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::701133005a11fb8cbc32d19df309669f
https://doi.org/10.1101/2020.10.01.322834
https://doi.org/10.1101/2020.10.01.322834
Autor:
Jelmer Cnossen, Marijn E. Siemons, Carlas Smith, Rasmus Ø. Thorsen, Ralf Jungmann, Taylor Hinsdale, Bernd Rieger, Sjoerd Stallinga, Florian Schueder
Publikováno v:
Nature methods
Nature Methods
Nature Methods (online), 17(1)
Nature Methods
Nature Methods (online), 17(1)
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extrac
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ff640a42c52c6279a22f08f81661a3f
https://doi.org/10.1038/s41592-019-0657-7
https://doi.org/10.1038/s41592-019-0657-7
Autor:
Ralf Jungmann, Taylor Hinsdale, Rasmus Ø. Thorsen, Jelmer Cnossen, Bernd Rieger, Carlas Smith, Sjoerd Stallinga, Florian Schueder
MINFLUX offers a breakthrough in single molecule localization precision, but suffers from a tiny field-of-view and a lack of practical parallelism. Here, we combine centroid estimation and illumination pattern induced photon count variations in a con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f9f08d91963c78e362a6bbac1f589df
Autor:
Rasmus Ø. Thorsen, Sjoerd Stallinga, Mathias Hammer, Christiaan N. Hulleman, David Grunwald, Bernd Rieger
Publikováno v:
nature methods
Nature Methods (online), 15(12)
Nature Methods (online), 15(12)
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d98bccb0dad45d30b446bec138148b17
https://pubmed.ncbi.nlm.nih.gov/30504874
https://pubmed.ncbi.nlm.nih.gov/30504874
Autor:
Sjoerd Stallinga, Hammer Mathias, Christiaan N. Hulleman, Bernd Rieger, Rasmus Ø. Thorsen, David Grunwald
Recently, Franke, Sauer and van de Linde1 introduced a way to estimate the axial position of single-molecules (TRABI). To this end, they compared the detected photon count from a temporal radial-aperture-based intensity estimation to the estimated co
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2c465b79b0729fa875dee8df201524dd
https://doi.org/10.1101/396424
https://doi.org/10.1101/396424
Publikováno v:
Single Molecule Spectroscopy and Superresolution Imaging XI.
In Single Molecule Localization Microscopy (SMLM) emission spots are fitted with a Point Spread Function (PSF) model in order to find the position of the molecules. Recently Franke et al. [Nature Methods 2017] found that the use of a Gaussian PSF mod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c90f926356171c770d1dc419905ce481
https://doi.org/10.1117/12.2289643
https://doi.org/10.1117/12.2289643
Autor:
Samel Arslanagic, Rasmus Ø. Thorsen
Publikováno v:
Photonics, Vol 2, Iss 3, Pp 773-794 (2015)
Photonics
Volume 2
Issue 3
Pages 773-794
Photonics
Volume 2
Issue 3
Pages 773-794
The present work shows how the eccentricity in active nano-particles may lead to very interesting and rather directive near- and far-field radiation patterns. The nano-particle is of a three-layer type and consists of a silica core, a free-space midd
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0e7b2e1cc6efaab7cd2f52e1a3a4d505
http://www.mdpi.com/2304-6732/2/3/773
http://www.mdpi.com/2304-6732/2/3/773