Zobrazeno 1 - 5
of 5
pro vyhledávání: '"Raphaela B. Liebherr"'
Autor:
Raphaela B. Liebherr, Hans H. Gorris
Publikováno v:
Molecules, Vol 19, Iss 9, Pp 14417-14445 (2014)
Large arrays of homogeneous microwells each defining a femtoliter volume are a versatile platform for monitoring the substrate turnover of many individual enzyme molecules in parallel. The high degree of parallelization enables the analysis of a stat
Externí odkaz:
https://doaj.org/article/8953289f14ae42f08208db537596eb4a
Autor:
Alfred Prof. Dr. Lechner, Helmut Hummel, Andrea Beutner, Hans H. Gorris, Raphaela B. Liebherr, Albert Hutterer, Matthias Jürgen Mickert, Franziska C. Vogl
Publikováno v:
Analytical and bioanalytical chemistry. 407(24)
Large arrays of femtoliter-sized chambers are important tools for single molecule research as well as bioanalytical applications. We have optimized the design and fabrication of two array types consisting of 250 × 250 (62 500) femtoliter chambers ei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71df817e20718b9f1d55876ff598869f
https://pubmed.ncbi.nlm.nih.gov/26253226
https://pubmed.ncbi.nlm.nih.gov/26253226
Publikováno v:
Journal of the American Chemical Society. 136(16)
The mechanisms that drive the evolution of new enzyme activity have been investigated by comparing the kinetics of wild-type and in vitro evolved β-glucuronidase (GUS) at the single molecule level. Several hundred single GUS molecules were separated
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::352487ca51b779760a37f206711d479b
https://pubmed.ncbi.nlm.nih.gov/24684619
https://pubmed.ncbi.nlm.nih.gov/24684619
Publikováno v:
Nanotechnology. 23(48)
Photon upconverting nanoparticles (UCNPs) have become an important new class of optical labels. Their unique property of emitting visible light after photo-excitation with near-infrared radiation enables biological imaging without background interfer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7aca49a3907191274e33b18605d0fdb
https://pubmed.ncbi.nlm.nih.gov/23128671
https://pubmed.ncbi.nlm.nih.gov/23128671
Publikováno v:
The Analyst. 138:4260
Large arrays of femtoliter-sized chambers were etched into the surface of fused silica slides to enclose and observe hundreds of single horseradish peroxidase (HRP) molecules in parallel. Individual molecules of HRP oxidize the fluorogenic substrate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b894d3bc89c2cd6c4075b99c9abc6984
https://doi.org/10.1039/c3an00809f
https://doi.org/10.1039/c3an00809f