Transfer of continuous manufacturing process principles for mAb production in a GMP environment: A step in the transition from batch to continuous

Autor: Kevin Botelho Ferreira, Asma Benlegrimet, Gabriel Diane, Victor Pasquier, Raphael Guillot, Marc De Poli, Loïc Chappuis, Nandita Vishwanathan, Jonathan Souquet, Hervé Broly, Jean‐Marc Bielser

Publikováno v: Biotechnology progressREFERENCES. 38(4)

Implementation of continuous in lieu of batch upstream processing (USP) and downstream process (DSP) for the production of recombinant therapeutic protein is a significant paradigm change. The present report describes how the first kilograms of monoc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::85b179f78119799f185aa5c244e4a58d
https://pubmed.ncbi.nlm.nih.gov/35412696

Effects of pyruvate on primary metabolism and product quality for a high-density perfusion process

Autor: Stefania Caso, Mathieu Aeby, Martin Jordan, Raphael Guillot, Jean‐Marc Bielser

Publikováno v: Biotechnology and bioengineeringREFERENCES. 119(4)

High volumetric productivities can be achieved when perfusion processes are operated at high cell densities. Yet it is fairly challenging to keep high cell density cultures in a steady state over an extended period. Aiming for robust processes, cultu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f26c5e7598e0f1ebd830fa3f44c39cc
https://pubmed.ncbi.nlm.nih.gov/35023143

Myoconductive and osteoinductive free-standing polysaccharide membranes

Autor: Jonathan Lavaud, Jean-Luc Coll, João F. Mano, Catherine Picart, Claire Monge, Sofia G. Caridade, Raphael Guillot, Jorge Almodovar, Véronique Josserand

Publikováno v: Acta Biomaterialia
Acta Biomaterialia, Elsevier, 2015, 15, pp.139-149. ⟨10.1016/j.actbio.2014.12.027⟩
Repositório Científico de Acesso Aberto de Portugal
Repositório Científico de Acesso Aberto de Portugal (RCAAP)
instacron:RCAAP

Free-standing (FS) membranes have increasing applications in the biomedical field as drug delivery systems for wound healing and tissue engineering. Here, we studied the potential of free-standing membranes made by the layer-by-layer assembly of chit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2e6eaa92016ce0aa84d31b7db34028d2
https://doi.org/10.1016/j.actbio.2014.12.027

The stability of BMP loaded polyelectrolyte multilayer coatings on titanium

Autor: Flora Gilde, Frédéric Sailhan, Delphine Logeart-Avramoglou, Catherine Picart, Aurélien Lapeyrere, Pierre Becquart, Raphael Guillot

Publikováno v: Biomaterials
Biomaterials, Elsevier, 2013, 34 (23), pp.5737-5746. ⟨10.1016/j.biomaterials.2013.03.067⟩

International audience; Immobilization of bone morphogenetic proteins (BMP) onto material surfaces is a promising, but still challenging, strategy for achieving dependable and consistent osseointegration of long-term metal implants. In the present st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d83218b3526732ba368f9018e059e0c
https://doi.org/10.1016/j.biomaterials.2013.03.067

Surface delivery of tunable doses of BMP-2 from an adaptable polymeric scaffold induces volumetric bone regeneration

Autor: Raphael Guillot, Georges Bettega, Jean Boutonnat, Cécile Olivier, Françoise Peyrin, Cedric Plettinx, Jean-Luc Coll, M. Bouyer, Véronique Curry, Catherine Picart, Véronique Josserand, Jonathan Lavaud

Publikováno v: Biomaterials
Biomaterials, Elsevier, 2016, 104, pp.168-181. ⟨10.1016/j.biomaterials.2016.06.001⟩

International audience; The rapid and effective bone regeneration of large non-healing defects remains challenging. Bioactive proteins, such as bone morphogenetic protein (BMP)-2, are proved their osteoinductivity, but their clinical use is currently

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f37b3ba61efdfc1ae4e04ab104bec3c
https://hal.archives-ouvertes.fr/hal-01376574