Transmembrane signaling by a synthetic receptor in artificial cells

Autor: Ane Bretschneider Søgaard, Andreas Bøtker Pedersen, Kaja Borup Løvschall, Pere Monge, Josefine Hammer Jakobsen, Leila Džabbarova, Line Friis Nielsen, Sandra Stevanovic, Raoul Walther, Alexander N. Zelikin

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-10 (2023)

Transmembrane signaling is the core adaptation in nature that allows cells to communicate. Here, the authors engineer signaling through the lipid bilayer using chemical, synthetic receptors for their use in the design of artificial cells.

Externí odkaz: https://doaj.org/article/d9661349c54041f0b7cda27311cb5580

Identification of macrocyclic peptides which activate bacterial cylindrical proteases

Autor: Raoul Walther, Linda M. Westermann, Sheiliza Carmali, Sophie E. Jackson, Heike Brötz-Oesterhelt, David R. Spring

Publikováno v: RSC Medicinal Chemistry.

The caseinolytic protease complex ClpXP is an important house-keeping enzyme in prokaryotes charged with the removal and degradation of misfolded and aggregated proteins and performing regulatory proteolysis. Dysregulation of its function, particular

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22093be07bed34b17f22d1b0c98fcb4a
https://doi.org/10.1039/d3md00136a

Per‐glycosylation of the Surface‐Accessible Lysines: One‐Pot Aqueous Route to Stabilized Proteins with Native Activity

Autor: Azad Farzadfard, Ole Aalund Mandrup, Pere Monge, Andreas Bøtker Pedersen, Alexander N. Zelikin, Anja Benderoth, Daniel E. Otzen, Kenneth A. Howard, Raoul Walther, Jannik Nedergaard Pedersen

Publikováno v: Walther, R, Monge, P, Pedersen, A, Benderoth, A, Pedersen, J, Farzadfard, A, Mandrup, O, Howard, K, Otzen, D & Zelikin, A N 2021, ' Per-glycosylation of the Surface-Accessible Lysines : One-Pot Aqueous Route to Stabilized Proteins with Native Activity ', ChemBioChem, vol. 22, no. 14, pp. 2478-2485 . https://doi.org/10.1002/cbic.202100228

Chemical glycosylation of proteins is a powerful tool applied widely in biomedicine and biotechnology. However, it is a challenging undertaking and typically relies on recombinant proteins and site-specific conjugations. The scope and utility of this

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7e867abcf5bcb80d38b2f009e217930
https://doi.org/10.1002/cbic.202100228

Extended scaffold glucuronides: en route to the universal synthesis of O-aryl glucuronide prodrugs

Autor: Alexander N. Zelikin, Raoul Walther, Morten T. Jarlstad Olesen

Publikováno v: Walther, R, Olesen, M T J & Zelikin, A N 2019, ' Extended scaffold glucuronides : en route to the universal synthesis of O-aryl glucuronide prodrugs ', Organic & Biomolecular Chemistry, vol. 17, no. 29, pp. 6970-6974 . https://doi.org/10.1039/c9ob01384a

We demonstrate that an extended scaffold based on a self-immolative linker (SIL) enables the universal production of O-aryl glucuronide prodrugs: high yield glucuronidation is performed on a precursor substrate (SIL) and the subsequent drug conjugati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f4c9cfbba33c42b3d5bc34d28f5b1be
https://doi.org/10.1039/c9ob01384a

Chemical (neo)glycosylation of biological drugs

Autor: Alexander N. Zelikin, Raoul Walther

Publikováno v: Walther, R & Zelikin, A N 2021, ' Chemical (neo)glycosylation of biological drugs ', Advanced Drug Delivery Reviews, vol. 171, pp. 62-76 . https://doi.org/10.1016/j.addr.2021.01.021

Biological drugs, specifically proteins and peptides, are a privileged class of medicinal agents and are characterized with high specificity and high potency of therapeutic activity. However, biologics are fragile and require special care during stor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::781288f11ea8ab89a9e44549d21b25eb
https://pure.au.dk/portal/da/publications/chemical-neoglycosylation-of-biological-drugs(cb7c804a-ab4f-466c-989b-0267c789a4bf).html