Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Randy Ira Hecht"'
Autor:
Dwight Winters, Todd Hager, Michael Hall, Shanaka Stanislaus, Murielle M. Véniant, Randy Ira Hecht, Chris Spahr, Lei Zhou, Jing Xu, Wei Wang, Yang Li, Junming Yie, Yue-Sheng Li, Liying Deng, Jennifer Weiszmann, Stephen Smith
Publikováno v:
Endocrinology. 158:1314-1327
Fibroblast growth factor (FGF) 21 is a natural hormone that modulates glucose, lipid, and energy metabolism. Previously, we engineered an Fc fusion FGF21 variant with two mutations, Fc-FGF21(RG), to extend the half-life and reduce aggregation and in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31e301d1ddf343756228eca215f2028d
https://doi.org/10.1210/en.2016-1917
https://doi.org/10.1210/en.2016-1917
Autor:
Minghan Wang, Lei-Ting Tam, Michelle Chen, Jing Xu, Liying Deng, Richard A. Lindberg, Yang Li, Wei Wang, Murielle M. Véniant, Randy Ira Hecht, Ching Chen, Jennitte Stevens, Junming Yie
Publikováno v:
Chemical Biology & Drug Design. 79:398-410
The endocrine fibroblast growth factor 21 (FGF21) requires both fibroblast growth factor receptor (FGFR) and β-Klotho for signaling. In this study, we sought to understand the inter-molecular physical interactions in the FGF21/FGFR/β-Klotho complex
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1fc59b101da505874682b804051bbbab
https://doi.org/10.1111/j.1747-0285.2012.01325.x
https://doi.org/10.1111/j.1747-0285.2012.01325.x
Autor:
Murielle M. Véniant, Jin-Long Chen, Clarence Hale, Richard A. Lindberg, Jing Xu, Zhiyou Zhang, Steven Vonderfecht, Glenn Sivits, Randy Ira Hecht, Yue-Sheng Li, Dae Young Jung, Shanaka Stanislaus, Hwi Jin Ko, Jason K. Kim, David Lloyd, Michelle Chen
Publikováno v:
Diabetes
OBJECTIVE—Fibroblast growth factor 21 (FGF21) has emerged as an important metabolic regulator of glucose and lipid metabolism. The aims of the current study are to evaluate the role of FGF21 in energy metabolism and to provide mechanistic insights
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18d223cd5ccdfcc94dd9132dbdfb095d
http://europepmc.org/articles/PMC2606881
http://europepmc.org/articles/PMC2606881
Publikováno v:
Process Biochemistry. 41:2473-2476
Screening protein molecules for a particular biological activity sometimes requires the purification of hundreds of proteins. Often many constructs, from several 100 to a 1000, are screened before the lead candidate is found. What is needed is a rapi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::665c114a474a8665e398ba309c6db21c
https://doi.org/10.1016/j.procbio.2006.06.010
https://doi.org/10.1016/j.procbio.2006.06.010
Autor:
Dwight Winters, Denis Healy, Mary Jane Cullen, Mary Ann Pelleymounter, Randy Ira Hecht, Michael McCaleb
Publikováno v:
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 275:R950-R959
Leptin efficacy was compared in obese and lean female CD-1 mice. Body weights in these 10- to 12-mo-old mice ranged from 29.7 to 62.0 g, and leptin levels correlated with body weight. Mice from the lean and obese ends of the weight distribution were
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bc54c940f480a76597e22855176faa28
https://doi.org/10.1152/ajpregu.1998.275.4.r950
https://doi.org/10.1152/ajpregu.1998.275.4.r950
Autor:
Pamela Hunt, Thomas C. Boone, Esther S. Choi, Randy Ira Hecht, Martha M. Hokom, Timothy Grasel, Judy Faust, Randy Steinbrink, Erik Dwyer, John Wilson, David L. Lacey, Christopher G. Rowan, Olaf B Kinstler, Dwight Winters, Janet L. Nichol, Steve Kaufman
Publikováno v:
Blood. 86:4486-4492
Megakaryocyte growth and development factor (MGDF) is a potent inducer of megakaryopoiesis in vitro and thrombopoiesis in vivo. The effects of MGDF appear to be lineage-selective, making this cytokine an ideal candidate for use in alleviating clinica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3f0f30d3024a59b92edef90d8b0f2d8c
https://doi.org/10.1182/blood.v86.12.4486.bloodjournal86124486
https://doi.org/10.1182/blood.v86.12.4486.bloodjournal86124486
Autor:
Mary Ann Pelleymounter, Dwight Winters, Thomas C. Boone, Mary Beth Baker, Randy Ira Hecht, Frank H. Collins, Mary Jane Cullen
Publikováno v:
Science. 269:540-543
C57BL/6J mice with a mutation in the obese (ob) gene are obese, diabetic, and exhibit reduced activity, metabolism, and body temperature. Daily intraperitoneal injection of these mice with recombinant OB protein lowered their body weight, percent bod
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5fe3287c4c3f0349e43293497a52e0d8
https://doi.org/10.1126/science.7624776
https://doi.org/10.1126/science.7624776
Autor:
Lei Zhou, Jing Xu, Randy Ira Hecht, Russell Wada, Shanaka Stanislaus, Murielle M. Véniant, Katherine Ann Winters, Todd Hager, Renee Komorowski, Ping Chen
Publikováno v:
Endocrinology. 153(9)
Fibroblast growth factor 21 (FGF21), a hormone with short half-life, has consistently shown strong pharmacological efficacy. We first assessed the efficacy of murine recombinant FGF21 in C57BL6 lean mice for 5 wk. We then generated a long-acting FGF2
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f23b45b61462cf73aef5f7eb0836c8a2
https://pubmed.ncbi.nlm.nih.gov/22798348
https://pubmed.ncbi.nlm.nih.gov/22798348
Autor:
Ling Cai, Zhong Shen, Frank Abroson, Amir Porat, Lei-Ting Tony Tam, Randy Ira Hecht, Dwight Winters, Steve Smith
Publikováno v:
Preparative biochemistrybiotechnology. 42(4)
Strong ion-exchange protein chromatography is one of the most powerful and most common steps for protein purification in both discovery research and manufacturing. However, the demands on protein purification of early drug discovery and later stage m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f0337215349c803f945a1b33c66f23ad
https://pubmed.ncbi.nlm.nih.gov/22708809
https://pubmed.ncbi.nlm.nih.gov/22708809
Autor:
Dwight Winters, Steve Smith, Mark Berge, Eleanor Le, Zhongqi Zhang, Robert W. Hong, Mark Leo Michaels, Jennifer Liu, Katariina M. Hutterer, Frank Abroson, Ed Belouski, Randy Ira Hecht, Bhavana Shah, Hedieh Barkhordarian
Publikováno v:
Biotechnology and bioengineering. 109(11)
High levels of translational errors, both truncation and misincorporation in an Fc-fusion protein were observed. Here, we demonstrate the impact of several commercially available codon optimization services, and compare to a targeted strategy. Using
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f1d0922b62029d4aee7d1c2f7f3aea88
https://pubmed.ncbi.nlm.nih.gov/22573571
https://pubmed.ncbi.nlm.nih.gov/22573571