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Akademický článek

Strain Construction and Process Development for Efficient Recombinant Production of Mannuronan C-5 Epimerases in Hansenula polymorpha

Autor: Anne Tøndervik, Randi Aune, Adelheid Degelmann, Michael Piontek, Helga Ertesvåg, Gudmund Skjåk-Bræk, Håvard Sletta

Publikováno v: Frontiers in Plant Science, Vol 13 (2022)

Alginates are linear polysaccharides produced by brown algae and some bacteria and are composed of β-D-mannuronic acid (M) and α-L-guluronic acid (G). Alginate has numerous present and potential future applications within industrial, medical and ph

Externí odkaz: https://doaj.org/article/6089eaf848234416a9fd8200705de2e5

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Akademický článek

Exploiting Mannuronan C-5 Epimerases in Commercial Alginate Production

Autor: Anne Tøndervik, Olav A. Aarstad, Randi Aune, Susan Maleki, Philip D. Rye, Arne Dessen, Gudmund Skjåk-Bræk, Håvard Sletta

Publikováno v: Marine Drugs, Vol 18, Iss 11, p 565 (2020)

Alginates are one of the major polysaccharide constituents of marine brown algae in commercial manufacturing. However, the content and composition of alginates differ according to the distinct parts of these macroalgae and have a direct impact on the

Externí odkaz: https://doaj.org/article/f1ebc2b6a49a4202a2c1ab15a21003e5

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Strain Construction and Process Development for Efficient Recombinant Production of Mannuronan C-5 Epimerases in

Autor: Anne, Tøndervik, Randi, Aune, Adelheid, Degelmann, Michael, Piontek, Helga, Ertesvåg, Gudmund, Skjåk-Bræk, Håvard, Sletta

Publikováno v: Frontiers in plant science. 13

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d26fbf28bb30cd390285beb7c80a1441
https://pubmed.ncbi.nlm.nih.gov/35734252

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Exploiting mannuronan C-5 epimerases in commercial alginate production

Autor: Arne Dessen, Olav Andreas Aarstad, Gudmund Skjåk-Bræk, Randi Aune, Philip D. Rye, Susan Maleki, Håvard Sletta, Anne Tøndervik

Publikováno v: 18:565
Marine Drugs
Marine Drugs, Vol 18, Iss 565, p 565 (2020)
Volume 18
Issue 11

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::015bc3c6b48575d481b3a8a980445cbf
https://hdl.handle.net/11250/2729037

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Alginates induce differentiation and expression of CXCR7 and CXCL12/SDF-1 in human keratinocytes-The role of calcium

Autor: Håvard Sletta, Randi Aune, Jørgen Stenvik, Sverre H. Torp, Gudmund Skjåk-Bræk, Liv Ryan, Brita Pukstad, Wenche Iren Strand, Anne Tøndervik, Terje Espevik, Øystein Grimstad

Publikováno v: Journal of Biomedical Materials Research Part A. :2803-2812

Alginates from seaweed are used in chronic wound management, though the molecular and cellular effects of various alginate dressings are not well documented. We have developed ultrapure sodium-alginates from Pseudomonas fluorescens with different con

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::21d539be53defa7ea3b30e0864fc70cd
https://doi.org/10.1002/jbm.a.34219

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The Presence of N-Terminal Secretion Signal Sequences Leads to Strong Stimulation of the Total Expression Levels of Three Tested Medically Important Proteins during High-Cell-Density Cultivations of Escherichia coli

Autor: T. E. Vee Aune, Aina Nedal, G. Evensen, Håvard Sletta, Anne Tøndervik, Svein Valla, Trygve Brautaset, Sigrid Hakvåg, Randi Aune, Trond E. Ellingsen

Publikováno v: Applied and Environmental Microbiology. 73:906-912

Genetic optimizations to achieve high-level production of three different proteins of medical importance for humans, granulocyte-macrophage colony-stimulating factor (GM-CSF), interferon alpha 2b (IFN-α2b), and single-chain antibody variable fragmen

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac879afdaba08bae1e787eebd5680d96
https://doi.org/10.1128/aem.01804-06

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Role of the Pseudomonas fluorescens Alginate Lyase (AlgL) in Clearing the Periplasm of Alginates Not Exported to the Extracellular Environment

Autor: Karianne Bakkevig, Randi Aune, Svein Valla, Bjørn E. Christensen, Kristin Degnes, Trond E. Ellingsen, Helga Ertesvåg, Martin Gimmestad, Håvard Sletta

Publikováno v: Journal of Bacteriology. 187:8375-8384

Alginate is an industrially widely used polysaccharide produced by brown seaweeds and as an exopolysaccharide by bacteria belonging to the genera Pseudomonas and Azotobacter . The polymer is composed of the two sugar monomers mannuronic acid and gulu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b082574ae53eeec02089ab303a9af91b
https://doi.org/10.1128/jb.187.24.8375-8384.2005

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Nystatin Biosynthesis and Transport: nysH and nysG Genes Encoding a Putative ABC Transporter System in Streptomyces noursei ATCC 11455 Are Required for Efficient Conversion of 10-Deoxynystatin to Nystatin

Autor: Trond E. Ellingsen, Per Bruheim, Olga N. Sekurova, Sergey B. Zotchev, Hans Grasdalen, Randi Aune, Sven Even F. Borgos, Håvard Sletta

Publikováno v: Antimicrobial Agents and Chemotherapy. 49:4576-4583

The genes nysH and nysG , encoding putative ABC-type transporter proteins, are located at the flank of the nystatin biosynthetic gene cluster in Streptomyces noursei ATCC 11455. To assess the possible roles of these genes in nystatin biosynthesis, th

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::69b94a733f17ec219b9f3276073568ce
https://doi.org/10.1128/aac.49.11.4576-4583.2005

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Broad-Host-Range Plasmid pJB658 Can Be Used for Industrial-Level Production of a Secreted Host-Toxic Single-Chain Antibody Fragment in Escherichia coli

Autor: Trygve Brautaset, Trond Erik Vee Aune, Randi Aune, Sigrid Hakvåg, Svein Valla, H. Hellebust, Trond E. Ellingsen, Håvard Sletta, Aina Nedal

Publikováno v: Applied and Environmental Microbiology. 70:7033-7039

In industrial scale recombinant protein production it is often of interest to be able to translocate the product to reduce downstream costs, and heterologous proteins may require the oxidative environment outside of the cytoplasm for correct folding.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06b51ef6421d9659bd528dddce1dd220
https://doi.org/10.1128/aem.70.12.7033-7039.2004

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Nystatin biosynthesis and transport: nysH and nysG genes encoding a putative ABC transporter system in Streptomyces noursei ATCC 11455 are required for efficient conversion of 10-deoxynystatin to nystatin

Autor: Håvard, Sletta, Sven E F, Borgos, Per, Bruheim, Olga N, Sekurova, Hans, Grasdalen, Randi, Aune, Trond E, Ellingsen, Sergey B, Zotchev

Publikováno v: Antimicrobial agents and chemotherapy. 49(11)

The genes nysH and nysG, encoding putative ABC-type transporter proteins, are located at the flank of the nystatin biosynthetic gene cluster in Streptomyces noursei ATCC 11455. To assess the possible roles of these genes in nystatin biosynthesis, the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a900c9b9094573584359b72751473591
https://pubmed.ncbi.nlm.nih.gov/16251298

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