A hydrolyzed N-propionylthiosuccinimide linker is cleaved by metastable fragmentation, increasing reliability of conjugation site identification in conjugate vaccines.

Autor: Ramos-Bermúdez PE; Center for Genetic Engineering and Biotechnology, Havana, Cuba., Pousa S; Center for Genetic Engineering and Biotechnology, Havana, Cuba., Carvalho P; Carlos Chagas Institute/FioCruz Rua Professor Algacyr Munhoz Mader, Curitiba, Brazil., Brant RSC; Carlos Chagas Institute/FioCruz Rua Professor Algacyr Munhoz Mader, Curitiba, Brazil., Batista M; Carlos Chagas Institute/FioCruz Rua Professor Algacyr Munhoz Mader, Curitiba, Brazil., Hojo H; Institute for Protein Research, Osaka University, Suita, Osaka, Japan., Garay HE; Center for Genetic Engineering and Biotechnology, Havana, Cuba., Roscoe A; Center for Genetic Engineering and Biotechnology, Havana, Cuba., Mallón AR; Center for Genetic Engineering and Biotechnology, Havana, Cuba., Besada V; Center for Genetic Engineering and Biotechnology, Havana, Cuba., Takao T; Institute for Protein Research, Osaka University, Suita, Osaka, Japan., González LJ; Carlos Chagas Institute/FioCruz Rua Professor Algacyr Munhoz Mader, Curitiba, Brazil.

Publikováno v: Rapid communications in mass spectrometry : RCM [Rapid Commun Mass Spectrom] 2024 Sep 30; Vol. 38 (18), pp. e9859.