Zobrazeno 1 - 10
of 100
pro vyhledávání: '"Ramona Schlesinger"'
Autor:
Kenichi Ataka, Axel Baumann, Jheng-Liang Chen, Aoife Redlich, Joachim Heberle, Ramona Schlesinger
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
The translocon-unassisted folding process of transmembrane domains of the microbial rhodopsins sensory rhodopsin I (HsSRI) and II (HsSRII), channelrhodopsin II (CrChR2), and bacteriorhodopsin (HsBR) during cell-free expression has been investigated b
Externí odkaz:
https://doaj.org/article/4e122f03c65640c283e3b82479595cca
Autor:
Fucsia Crea, Antreas Vorkas, Aoife Redlich, Rubén Cruz, Chaowei Shi, Dirk Trauner, Adam Lange, Ramona Schlesinger, Joachim Heberle
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
Optogenetics in the conventional sense, i.e. the use of engineered proteins that gain their light sensitivity from naturally abundant chromophores, represents an exciting means to trigger and control biological activity by light. As an alternate appr
Externí odkaz:
https://doaj.org/article/252058baea61487ba4001928c8af6fab
Autor:
Federico Baserga, Antreas Vorkas, Fucsia Crea, Luiz Schubert, Jheng-Liang Chen, Aoife Redlich, Mariafrancesca La Greca, Julian Storm, Sabine Oldemeyer, Kirsten Hoffmann, Ramona Schlesinger, Joachim Heberle
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
It is well known that lipids neighboring integral membrane proteins directly influence their function. The opposite effect is true as well, as membrane proteins undergo structural changes after activation and thus perturb the lipidic environment. Her
Externí odkaz:
https://doaj.org/article/83fc5add385e4b87ac5fa2629ced1454
Autor:
Mariafrancesca La Greca, Jheng-Liang Chen, Luiz Schubert, Jacek Kozuch, Tim Berneiser, Ulrich Terpitz, Joachim Heberle, Ramona Schlesinger
Publikováno v:
Frontiers in Molecular Biosciences, Vol 9 (2022)
Microbial rhodopsins have recently been discovered in pathogenic fungi and have been postulated to be involved in signaling during the course of an infection. Here, we report on the spectroscopic characterization of a light-driven proton pump rhodops
Externí odkaz:
https://doaj.org/article/d3846136bbf5460ba8359fa9f5053c66
Publikováno v:
Frontiers in Molecular Biosciences, Vol 8 (2022)
Direct optical activation of microbial rhodopsins in deep biological tissue suffers from ineffective light delivery because visible light is strongly scattered and absorbed. NIR light has deeper tissue penetration, but NIR-activation requires a trans
Externí odkaz:
https://doaj.org/article/fc07b089b1d34258a922c7a3d7ee2b6f
Autor:
Gabriela Nass Kovacs, Jacques-Philippe Colletier, Marie Luise Grünbein, Yang Yang, Till Stensitzki, Alexander Batyuk, Sergio Carbajo, R. Bruce Doak, David Ehrenberg, Lutz Foucar, Raphael Gasper, Alexander Gorel, Mario Hilpert, Marco Kloos, Jason E. Koglin, Jochen Reinstein, Christopher M. Roome, Ramona Schlesinger, Matthew Seaberg, Robert L. Shoeman, Miriam Stricker, Sébastien Boutet, Stefan Haacke, Joachim Heberle, Karsten Heyne, Tatiana Domratcheva, Thomas R. M. Barends, Ilme Schlichting
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-17 (2019)
Bacteriorhodopsin (bR) is a light-driven proton pump. Here the authors combine time-resolved crystallography at a free-electron laser, ultrafast spectroscopy and quantum chemistry to study the structural changes following multiphoton photoexcitation
Externí odkaz:
https://doaj.org/article/a086ef8dd24c43efbb5f1448e1c960cf
Autor:
Nicola J. Harris, Eamonn Reading, Kenichi Ataka, Lucjan Grzegorzewski, Kalypso Charalambous, Xia Liu, Ramona Schlesinger, Joachim Heberle, Paula J. Booth
Publikováno v:
Scientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
Abstract Correctly folded membrane proteins underlie a plethora of cellular processes, but little is known about how they fold. Knowledge of folding mechanisms centres on reversible folding of chemically denatured membrane proteins. However, this can
Externí odkaz:
https://doaj.org/article/8f8480ca6af2428ea155c29546970aae
Autor:
Gabriela Kovácsová, Marie Luise Grünbein, Marco Kloos, Thomas R. M. Barends, Ramona Schlesinger, Joachim Heberle, Wolfgang Kabsch, Robert L. Shoeman, R. Bruce Doak, Ilme Schlichting
Publikováno v:
IUCrJ, Vol 4, Iss 4, Pp 400-410 (2017)
Serial (femtosecond) crystallography at synchrotron and X-ray free-electron laser (XFEL) sources distributes the absorbed radiation dose over all crystals used for data collection and therefore allows measurement of radiation damage prone systems, in
Externí odkaz:
https://doaj.org/article/6328d4fa858149478ab0c0ff8fb3de4a
Autor:
Mirko Stauffer, Stephan Hirschi, Zöhre Ucurum, Daniel Harder, Ramona Schlesinger, Dimitrios Fotiadis
Publikováno v:
Methods and Protocols, Vol 3, Iss 3, p 51 (2020)
The light-driven proton pump bacteriorhodopsin (BR) from the extreme halophilic archaeon Halobacterium salinarum is a retinal-binding protein, which forms highly ordered and thermally stable 2D crystals in native membranes (termed purple membranes).
Externí odkaz:
https://doaj.org/article/d7f1aa4c173a49d19ae0fb20b0a831ca
Publikováno v:
Molecules, Vol 25, Iss 4, p 848 (2020)
Channelrhodopsins (ChR) are light-gated ion-channels heavily used in optogenetics. Upon light excitation an ultrafast all-trans to 13-cis isomerization of the retinal chromophore takes place. It is still uncertain by what means this reaction leads to
Externí odkaz:
https://doaj.org/article/9f87733b4fb843b6ab98aff200e3f920