Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Ramona Gruetzner"'
Peripheral infrastructure vectors and an extended set of plant parts for the Modular Cloning system.
Autor:
Johannes Gantner, Jana Ordon, Theresa Ilse, Carola Kretschmer, Ramona Gruetzner, Christian Löfke, Yasin Dagdas, Katharina Bürstenbinder, Sylvestre Marillonnet, Johannes Stuttmann
Publikováno v:
PLoS ONE, Vol 13, Iss 5, p e0197185 (2018)
Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type II
Externí odkaz:
https://doaj.org/article/406ba302ecbb4d6485a8348c207c23d7
Publikováno v:
PLoS ONE, Vol 6, Iss 2, p e16765 (2011)
The field of synthetic biology promises to revolutionize biotechnology through the design of organisms with novel phenotypes useful for medicine, agriculture and industry. However, a limiting factor is the ability of current methods to assemble compl
Externí odkaz:
https://doaj.org/article/3f13e5f5be6347998ec5644eed1d05e9
Publikováno v:
PLoS ONE, Vol 6, Iss 5, p e19722 (2011)
Generation of customized DNA binding domains targeting unique sequences in complex genomes is crucial for many biotechnological applications. The recently described DNA binding domain of the transcription activator-like effectors (TALEs) from Xanthom
Externí odkaz:
https://doaj.org/article/c54548b79ce44e05b14f695fea016465
Publikováno v:
PLoS ONE, Vol 4, Iss 5, p e5553 (2009)
We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the use of ty
Externí odkaz:
https://doaj.org/article/1ed45db2e77448e0907590df9cc03165
Autor:
Ramona Gruetzner, Pascal Püllmann, Steffen Neumann, Chris Ulpinnis, Martin J. Weissenborn, Sylvestre Marillonnet
Publikováno v:
Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
Scientific Reports
Scientific Reports
Site-directed methods for the generation of genetic diversity are essential tools in the field of directed enzyme evolution. The Golden Gate cloning technique has been proven to be an efficient tool for a variety of cloning setups. The utilization of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e8dbb249983e62996ad3a6a12c06ad08
Autor:
Sylvestre Marillonnet, Theresa Ilse, Carola Kretschmer, Jana Ordon, Johannes Stuttmann, Johannes Gantner, Katharina Bürstenbinder, Christian Löfke, Ramona Gruetzner, Yasin F. Dagdas
Publikováno v:
PLoS ONE, Vol 13, Iss 5, p e0197185 (2018)
PLoS ONE
PLoS ONE
Standardized DNA assembly strategies facilitate the generation of multigene constructs from collections of building blocks in plant synthetic biology. A common syntax for hierarchical DNA assembly following the Golden Gate principle employing Type II
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eef2394227b08fb8f62a546e8d213813
http://europepmc.org/articles/PMC5976141?pdf=render
http://europepmc.org/articles/PMC5976141?pdf=render
Autor:
Sylvestre Marillonnet, Nicola J. Patron, Tim-Martin Ehnert, Jonathan D. G. Jones, Ramona Gruetzner, Stefan Werner, Mark Youles, Carola Engler
Publikováno v:
ACS Synthetic Biology. 3:839-843
Plant Synthetic Biology requires robust and efficient methods for assembling multigene constructs. Golden Gate cloning provides a precision module-based cloning technique for facile assembly of multiple genes in one construct. We present here a versa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::751d77682f0c895a080ac36b3d58a298
https://doi.org/10.1021/sb4001504
https://doi.org/10.1021/sb4001504
Publikováno v:
Bioengineered. 3:38-43
Recent progress in the field of synthetic biology has led to the creation of cells containing synthetic genomes. Although these first synthetic organisms contained copies of natural genomes, future work will be directed toward engineering of organism
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29424befdb77216041068430f2fb3d91
https://doi.org/10.4161/bbug.3.1.18223
https://doi.org/10.4161/bbug.3.1.18223
Publikováno v:
PLoS ONE
PLoS ONE, Vol 4, Iss 5, p e5553 (2009)
PLoS ONE, Vol 4, Iss 5, p e5553 (2009)
We have developed a protocol to assemble in one step and one tube at least nine separate DNA fragments together into an acceptor vector, with 90% of recombinant clones obtained containing the desired construct. This protocol is based on the use of ty
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34f3d0ac2ecf550cb9184911bf5d54dc
https://pubmed.ncbi.nlm.nih.gov/19436741
https://pubmed.ncbi.nlm.nih.gov/19436741
Publikováno v:
PLoS ONE
PLoS ONE, Vol 6, Iss 2, p e16765 (2011)
PLoS ONE, Vol 6, Iss 2, p e16765 (2011)
The field of synthetic biology promises to revolutionize biotechnology through the design of organisms with novel phenotypes useful for medicine, agriculture and industry. However, a limiting factor is the ability of current methods to assemble compl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b700e84f7329f0009eabcd1c13efcf30
https://doi.org/10.1371/journal.pone.0016765
https://doi.org/10.1371/journal.pone.0016765