Zobrazeno 1 - 10
of 120
pro vyhledávání: '"Ralph Scully"'
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101551- (2022)
Summary: In this protocol, we use CRISPR/Cas9 to generate large deletions of the entire coding region of a gene of interest, generating a hemizygous cell line. Next, we systematically engineer precise in-frame deletions within the intact wild-type al
Externí odkaz:
https://doaj.org/article/cf04c21040b54664ad092286e10ec344
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101529- (2022)
Summary: Chromatin immunoprecipitation coupled with quantitative PCR (ChIP-qPCR) even with optimization may give low signal-to-background ratio and spatial resolution. Here, we adapted Cleavage Under Targets and Release Using Nuclease (CUT&RUN) (orig
Externí odkaz:
https://doaj.org/article/43fea6294a2d47e9bfb4d57950c783a8
Publikováno v:
PLoS Genetics, Vol 14, Iss 7, p e1007486 (2018)
Classical non-homologous end joining (C-NHEJ) and homologous recombination (HR) compete to repair mammalian chromosomal double strand breaks (DSBs). However, C-NHEJ has no impact on HR induced by DNA nicking enzymes. In this case, the replication for
Externí odkaz:
https://doaj.org/article/7e4e077d23e2453286634fff59ee8868
Publikováno v:
PLoS Genetics, Vol 12, Iss 12, p e1006509 (2016)
[This corrects the article DOI: 10.1371/journal.pgen.1006410.].
Externí odkaz:
https://doaj.org/article/210aed2e8b624dcb849d1931fb182640
Publikováno v:
PLoS Genetics, Vol 12, Iss 11, p e1006410 (2016)
A proportion of homologous recombination (HR) events in mammalian cells resolve by "long tract" gene conversion, reflecting copying of several kilobases from the donor sister chromatid prior to termination. Cells lacking the major hereditary breast/o
Externí odkaz:
https://doaj.org/article/bef189905b1f4add967aa3293e0078f0
Autor:
Josée Guirouilh-Barbat, Camille Gelot, Anyong Xie, Elodie Dardillac, Ralph Scully, Bernard S Lopez
Publikováno v:
PLoS Genetics, Vol 12, Iss 10, p e1006230 (2016)
DNA double-strand breaks (DSB) are very harmful lesions that can generate genome rearrangements. In this study, we used intrachromosomal reporters to compare both the efficiency and accuracy of end-joining occurring with close (34 bp apart) vs. dista
Externí odkaz:
https://doaj.org/article/fb7a564808c345528c10708661e020aa
Autor:
Andrea J Hartlerode, Yinghua Guan, Anbazhagan Rajendran, Kiyoe Ura, Gunnar Schotta, Anyong Xie, Jagesh V Shah, Ralph Scully
Publikováno v:
PLoS ONE, Vol 7, Iss 11, p e49211 (2012)
Recruitment of 53BP1 to chromatin flanking double strand breaks (DSBs) requires γH2AX/MDC1/RNF8-dependent ubiquitination of chromatin and interaction of 53BP1 with histone H4 methylated on lysine 20 (H4K20me). Several histone methyltransferases have
Externí odkaz:
https://doaj.org/article/51153115b6584b1b91bea854d1bd5d6d
Autor:
Ralph Scully
Publikováno v:
PLoS Genetics, Vol 7, Iss 7, p e1002183 (2011)
Externí odkaz:
https://doaj.org/article/b6216461065e43219be1f97f37733150
Publikováno v:
PLoS ONE, Vol 6, Iss 3, p e16501 (2011)
Double-strand break repair is executed by two major repair pathways: non-homologous end joining (NHEJ) and homologous recombination (HR). Whereas NHEJ contributes to the repair of ionizing radiation (IR)-induced double strand breaks (DSBs) throughout
Externí odkaz:
https://doaj.org/article/bcae279ba6734e82a01c328ec9a186b2
Autor:
Rajula Elango, Arvind Panday, Francis P. Lach, Nicholas A. Willis, Kaitlin Nicholson, Erin E. Duffey, Agata Smogorzewska, Ralph Scully
Publikováno v:
Nat Struct Mol Biol
Vertebrate replication forks arrested at interstrand DNA cross-links (ICLs) engage the Fanconi anemia pathway to incise arrested forks, 'unhooking' the ICL and forming a double strand break (DSB) that is repaired by homologous recombination (HR). The
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b21c2311ab2f1f53752438a58784c07a
https://doi.org/10.1038/s41594-022-00812-9
https://doi.org/10.1038/s41594-022-00812-9