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pro vyhledávání: '"Ralph L. McAnelly"'
Autor:
Sandrine Legault, Derek P. Fraser-Halberg, Ralph L. McAnelly, Matthew G. Eason, Michael C. Thompson, Roberto A. Chica
Publikováno v:
Chemical Science. 13:1408-1418
We used computational design to increase quantum yield in a fluorescent protein by optimizing chromophore packing to reduce non-radiative decay, resulting in an >10-fold increase in quantum yield that was further improved by directed evolution.
Autor:
Sandrine, Legault, Derek P, Fraser-Halberg, Ralph L, McAnelly, Matthew G, Eason, Michael C, Thompson, Roberto A, Chica
Publikováno v:
Chemical science. 13(5)
Red fluorescent proteins (RFPs) have found widespread application in chemical and biological research due to their longer emission wavelengths. Here, we use computational protein design to increase the quantum yield and thereby brightness of a dim mo