Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Ralph J. Cisneros"'
Publikováno v:
Journal of Biological Chemistry. 268:10102-10108
The binding of 5-fluorodeoxyuridylate (FdUMP) to carboxypeptidase-inactivated thymidylate synthase obtained from methotrexate-resistant Lactobacillus casei was investigated using [3H]FdUMP in a trichloroacetic acid precipitation assay and by 19F nucl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a1a2bc2b694863463b5b905d80944821
https://doi.org/10.1016/s0021-9258(18)82177-9
https://doi.org/10.1016/s0021-9258(18)82177-9
Autor:
R. Bruce Dunlap, Jerome D. Odom, Ralph J. Cisneros, Mary S. Weir, Jeffrey O. Boles, J. E. Villafranca
Publikováno v:
Biochemistry. 30:11073-11080
Replacement of methionine (Met) residues by selenomethionine (SeMet) was recently shown to facilitate the crystallographic analysis of protein structure through the application of multi-wavelength anomalous diffraction techniques [Yang et al. (1990)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61c71795277d2b48a4698573ba07d57d
https://doi.org/10.1021/bi00110a009
https://doi.org/10.1021/bi00110a009
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1077:35-46
Recombinant mouse thymidylate synthase (TS) expressed at high levels in Escherichia coli was purified to homogeneity in greater than 70% yield by a rapid three-step procedure. Both 0.1% Triton X-100 and 10% glycerol were required to stabilize the enz
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6e5715ddf4fc74b1054caa55657833c9
https://doi.org/10.1016/0167-4838(91)90523-3
https://doi.org/10.1016/0167-4838(91)90523-3
Publikováno v:
Zurich, Switzerland, September 3–8, 1989
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::96134a49efe0632d4d811ae14681b084
https://doi.org/10.1515/9783110889000-160
https://doi.org/10.1515/9783110889000-160
Autor:
Ralph J. Cisneros, R. Bruce Dunlap
Publikováno v:
Biochimica et biophysica acta. 1039(2)
Covalent binding stoichiometries for both the enzyme:5-fluoro-2'-deoxyuridine 5'-monophosphate (FdUMP) binary complex and the enzyme:FdUMP:5,10-methylenetetrahydrofolate (inhibitory ternary) complex at equilibrium were measured by the trichloroacetic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13a8ed22e1e9b56ca269f0c003f055d8
https://pubmed.ncbi.nlm.nih.gov/2114176
https://pubmed.ncbi.nlm.nih.gov/2114176
Autor:
Ralph J. Cisneros, R. Bruce Dunlap
Publikováno v:
Analytical biochemistry. 186(2)
The use of trichloroacetic acid as a protein precipitant and denaturant in the quantitative measurement of covalent complexes of thymidylate synthase is described. Enzyme inactivated with N[3H]ethylmaleimide and inhibitory ternary complex (formed wit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8c539732133e943337a3bd3161966315
https://pubmed.ncbi.nlm.nih.gov/2363490
https://pubmed.ncbi.nlm.nih.gov/2363490
Publikováno v:
Biochemical and biophysical research communications. 167(3)
The arginine located at position 44 of mouse thymidylate synthase is in a highly conserved loop that is in close proximity to the active site cleft of the enzyme. Structural analyses have suggested that this arginine forms hydrogen bonds with the α-
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e6c31076b3d79b2c965360e6bf2c78c
https://pubmed.ncbi.nlm.nih.gov/2322283
https://pubmed.ncbi.nlm.nih.gov/2322283
Publikováno v:
Gene. 84:487-491
The coding region of the mouse thymidylate synthase (TS)-encoding cDNA (ts) was inserted downstream from the phage T7 promoter and translation initiation signals of the expression vector, pET-3a, and transformed into Escherichia coli BL21(DE3)[pLysS]
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9adfe2d944e51926446d4b473d7044e
https://doi.org/10.1016/0378-1119(89)90525-8
https://doi.org/10.1016/0378-1119(89)90525-8
Publikováno v:
Nucleosides, Nucleotides and Nucleic Acids. 8:1135-1140
5-Methylselenenyl and 5-phenyltellurenyldeoxyuridines were constructed via the transmetallation of the corresponding 5-bromo-3′,5′-bis(dimethyl-tert-butyl)silyl deoxyuridine with n-butyllithium followed by electrophilic trapping of the anion with
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bbb6e5888bb918adee1564d40d1c4463
https://doi.org/10.1080/07328318908054310
https://doi.org/10.1080/07328318908054310