Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Ralf-Bernhardt Rues"'
Publikováno v:
Methods. 147:73-83
Cell-free production of G-protein coupled receptors is becoming attractive for biochemical characterization, ligand screening or even structural purposes. However, despite high production levels within the range of mg/mL, the fraction of functionally
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb09b6fdd459bf002101be100832da78
https://doi.org/10.1016/j.ymeth.2018.01.012
https://doi.org/10.1016/j.ymeth.2018.01.012
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Biomembranes. 1858:1306-1316
G protein-coupled receptors are of key significance for biomedical research. Streamlined approaches for their efficient recombinant production are of pivotal interest in order to explore their intrinsic conformational dynamics and complex ligand bind
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd2379f6f14c52dc8c96d96ccc6b7d86
https://doi.org/10.1016/j.bbamem.2016.02.031
https://doi.org/10.1016/j.bbamem.2016.02.031
Autor:
Robin Kleebach, Mariya Lazarova, Volker Dötsch, Frank Bernhard, Ralf-Bernhardt Rues, Frank Löhr
Publikováno v:
ACS chemical biology. 13(8)
NMR studies of large proteins are complicated by pronounced spectral overlap and large line width. Reducing complexity by [13C, 1H] selective labeling of l-Val, l-Leu, and/or l-Ile residues in combination with optional perdeuteration is therefore com
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8ab9cab415fffbbf0c219114e7f7994a
https://pubmed.ncbi.nlm.nih.gov/29894149
https://pubmed.ncbi.nlm.nih.gov/29894149
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1586
Cell-free expression allows to synthesize membrane proteins in completely new formats that can relatively easily be customized for particular applications. Amphiphilic superstructures such as micelles, lipomicelles, or nanodiscs can be provided as na
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::a11b92c7ddbe57a7bfb7f7d7bf478336
https://pubmed.ncbi.nlm.nih.gov/28470613
https://pubmed.ncbi.nlm.nih.gov/28470613
Publikováno v:
Methods in Molecular Biology ISBN: 9781493968855
Cell-free expression allows to synthesize membrane proteins in completely new formats that can relatively easily be customized for particular applications. Amphiphilic superstructures such as micelles, lipomicelles, or nanodiscs can be provided as na
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::961618bd890faced9b18516c0338a21a
https://doi.org/10.1007/978-1-4939-6887-9_19
https://doi.org/10.1007/978-1-4939-6887-9_19
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1432
The complexity of membrane protein synthesis is largely reduced in cell-free systems and it results into high success rates of target expression. Protocols for the preparation of bacterial lysates have been optimized in order to ensure reliable effic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::56a23376ea3e7d461cf778a3cc3160b5
https://pubmed.ncbi.nlm.nih.gov/27485326
https://pubmed.ncbi.nlm.nih.gov/27485326
Publikováno v:
Methods in Molecular Biology ISBN: 9781493936359
The complexity of membrane protein synthesis is largely reduced in cell-free systems and it results into high success rates of target expression. Protocols for the preparation of bacterial lysates have been optimized in order to ensure reliable effic
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::36150a07d85ea0e769ab46af65b74348
https://doi.org/10.1007/978-1-4939-3637-3_1
https://doi.org/10.1007/978-1-4939-3637-3_1
Autor:
Christine Gless, Michael Gerrits, Helmut Merk, Kerstin Beyer, Fang Dong, Frank Bernhard, Volker Dötsch, Ralf-Bernhardt Rues
Publikováno v:
Biological chemistry. 396(9-10)
G protein-coupled receptors, like many other membrane proteins, are notoriously difficult to synthesize in conventional cellular systems. Although expression in insect cells is considered the preferred technique for structural characterizations in pa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::662fbfafd5c6abd97c7f903580a76807
https://pubmed.ncbi.nlm.nih.gov/25999328
https://pubmed.ncbi.nlm.nih.gov/25999328
Publikováno v:
Biological chemistry. 395(12)
Based on their eminent importance for medical applications, G-protein coupled receptors are currently amongst the most frequently membrane protein targets analyzed by cell-free expression. The cell-free expression approach removes most bottlenecks kn
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::822534e739e267ab235bdb65efac5dd9
https://pubmed.ncbi.nlm.nih.gov/25178907
https://pubmed.ncbi.nlm.nih.gov/25178907