Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Ralf Ostendorp"'
Autor:
Meike Wolber, Marion Satzger, Daniela Della Ducata, Ralf Ostendorp, Cornelia Hanel, Jan Jaehrling, Stefan Pabst, Bodo Brocks
Publikováno v:
SLAS Discovery. 20:1256-1267
The generation of therapeutic antibodies with extremely high affinities down to the low picomolar range is today feasible with state-of-the art recombinant technologies. However, reliable and efficient identification of lead candidates with the desir
Autor:
Daniel Weinfurtner, Ralf Ostendorp, Roy Eylenstein, Stefan Steidl, Jark Böttcher, Stefan Härtle, Martin Augustin, Ralf Strohner
Publikováno v:
mAbs. 8(1)
X-ray structure analysis of 4 antibody Fab fragments, each in complex with human granulocyte macrophage colony stimulating factor (GM-CSF), was performed to investigate the changes at the protein-protein binding interface during the course of in vitr
Autor:
Titus Kretzschmar, Ralf Ostendorp, Bodo Brocks, Thomas von Rüden, Christian Frisch, Adolf Hoess
Publikováno v:
Journal of Immunological Methods. 275:203-212
We have developed a method for the high-level expression of expressed sequence tags (ESTs) as inclusion bodies in Escherichia coli by C-terminal fusion to the N1-domain of g3p of filamentous phage M13. Soluble fusion protein is obtained by an efficie
Autor:
Stefan Kaden, Stefan Pabst, Jan Jaehrling, Mechthild Linnemann, Karin Felderer, Anne Höhne, Sebastian Jäger, Bodo Brocks, Daniela Daubert, Tobias Neuber, Thomas Tiller, Johanna Kölln, Ralf Ostendorp, Katrin Frese
Publikováno v:
mAbs. 6(4)
The neonatal Fc receptor (FcRn) protects immunoglobulin G (IgG) from degradation and increases the serum half-life of IgG, thereby contributing to a higher concentration of IgG in the serum. Because altered FcRn binding may result in a reduced or pro
Autor:
Timothy L. Tellinghuisen, Richard J. Kuhn, Agnes E. Hamburger, Bonnie R. Fisher, Ralf Ostendorp
Publikováno v:
Journal of Virology. 73:5309-5319
The production of the alphavirus virion is a multistep event requiring the assembly of the nucleocapsid core in the cytoplasm and the maturation of the glycoproteins in the endoplasmic reticulum and the Golgi apparatus. These components associate dur
Autor:
Thomas Dams, Rainer Jaenicke, Ralf Ostendorp, Günter Auerbach, Ingo Korndörfer, Lars Prade, Robert Huber
Publikováno v:
Structure. 6(6):769-781
Background: L(+)-Lactate dehydrogenase (LDH) catalyzes the last step in anaerobic glycolysis, the conversion of pyruvate to lactate, with the concomitant oxidation of NADH. Extensive physicochemical and structural investigations of LDHs from both mes
Publikováno v:
mAbs. 5(2)
Antibody-based therapeutics are of great value for the treatment of human diseases. In addition to functional activity, affinity or physico-chemical properties, antibody specificity is considered to be one of the most crucial attributes for safety an
Publikováno v:
European Journal of Biochemistry. 240:274-279
Lactate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima has been functionally expressed in Escherichia coli. As shown by gel-permeation chromatography, dynamic light scattering, and ultracentrifugation, the recombinant protein
Publikováno v:
Protein Science. 5:862-873
L(+)-lactate dehydrogenase (LDH; E.C.1.1.1.27) from the hyperthermophilic bacterium Thermotoga maritima has been shown to represent the most stable LDH isolated so far (Wrba A, Jaenicke R, Huber R, Stetter KO, 1990, Eur J Biochem 188:195-201). In ord
Publikováno v:
Protein & Peptide Letters. 2:281-286
Abstract: The gene encoding 3-phosphoglycerate kinase (PGK) and triosephosphate isomerase (TIM) from the hyperthermophilic bacterium Thermotoga maritima has been cloned in E.coli. The gene consists of two overlapping open reading frames. In E.coli, a