Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Rakhi Rajan"'
Autor:
Wei Jiang, Jaideep Singh, Aleique Allen, Yue Li, Venkatesan Kathiresan, Omair Qureshi, Narin Tangprasertchai, Xiaojun Zhang, Hari Priya Parameshwaran, Rakhi Rajan, Peter Z. Qin
Publikováno v:
ACS Omega, Vol 4, Iss 17, Pp 17140-17147 (2019)
Externí odkaz:
https://doaj.org/article/4d9c0d4808a6404f85a8638771245c72
Publikováno v:
Frontiers in Cellular and Infection Microbiology, Vol 10 (2021)
Bacterial and archaeal CRISPR-Cas systems offer adaptive immune protection against foreign mobile genetic elements (MGEs). This function is regulated by sequence specific binding of CRISPR RNA (crRNA) to target DNA/RNA, with an additional requirement
Externí odkaz:
https://doaj.org/article/50076a2261ca4a96866a4f1ebb9067e6
Publikováno v:
Cell Reports, Vol 21, Iss 13, Pp 3728-3739 (2017)
CRISPR-Cas systems provide bacteria and archaea with sequence-specific protection against invading mobile genetic elements. In the presence of divalent metal ions, Cas9 and Cas12a (formerly Cpf1) proteins target and cleave DNA that is complementary t
Externí odkaz:
https://doaj.org/article/8f5a8f6dc6e24c118b47d10b06d12b5b
Autor:
Zhicheng Zuo, Ashwini Zolekar, Kesavan Babu, Victor JT Lin, Hamed S Hayatshahi, Rakhi Rajan, Yu-Chieh Wang, Jin Liu
Publikováno v:
eLife, Vol 8 (2019)
The CRISPR-associated endonuclease Cas9 from Streptococcus pyogenes (SpyCas9), along with a programmable single-guide RNA (sgRNA), has been exploited as a significant genome-editing tool. Despite the recent advances in determining the SpyCas9 structu
Externí odkaz:
https://doaj.org/article/b101c061019740009c2976871f7296dc
Publikováno v:
PeerJ, Vol 5, p e3161 (2017)
The Clustered Regularly Interspaced Short Palindromic Repeats associated (CRISPR-Cas) systems consist of RNA-protein complexes that provide bacteria and archaea with sequence-specific immunity against bacteriophages, plasmids, and other mobile geneti
Externí odkaz:
https://doaj.org/article/7e44169b05704809a390a77dce848fe5
Publikováno v:
Integrated Methods in Protein Biochemistry: Part B ISBN: 9780323992640
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::89d73eac6e21f761d5f31266f6eb82d4
https://doi.org/10.1016/bs.mie.2022.08.048
https://doi.org/10.1016/bs.mie.2022.08.048
Autor:
Pratibha Kumari, Peter Z. Qin, Xiongping Chen, Kesavan Babu, Venkatesan Kathiresan, Rakhi Rajan, Sydney Newsom, Jin Liu, Hari Priya Parameshwaran
Publikováno v:
Biochemistry. 60:3783-3800
CRISPR-Cas systems are RNA-guided nucleases that provide adaptive immune protection in bacteria and archaea against intruding genomic materials. Cas9, a type-II CRISPR effector protein, is widely used for gene editing applications since a single guid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e20300d5bb518c29518d846d83533579
https://doi.org/10.1021/acs.biochem.1c00354
https://doi.org/10.1021/acs.biochem.1c00354
Autor:
Tamara, Flusche, Rakhi, Rajan
Publikováno v:
Advances in experimental medicine and biology.
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins constitute an adaptive immune system in bacteria and archaea, where immunological memory is retained in the CRISPR locus as short pieces of the in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::fa3a752a37ca7f8cb791584c981a45c8
https://pubmed.ncbi.nlm.nih.gov/35852729
https://pubmed.ncbi.nlm.nih.gov/35852729
Autor:
Peter Z. Qin, Venkatesan Kathiresan, Kesavan Babu, Christine Tran, Hari Priya Parameshwaran, Kevin Guan, Rakhi Rajan, Aleique Allen
Publikováno v:
FEBS Lett
Cas12a is an RNA-guided DNA endonuclease of the type V-A CRISPR-Cas system that has evolved convergently with the type II Cas9 protein. We previously showed that proline substitutions in the bridge helix (BH) impart target DNA cleavage selectivity in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04f5b157de3f2cebc2303ef29f2b37c6
https://doi.org/10.1002/1873-3468.14051
https://doi.org/10.1002/1873-3468.14051
Autor:
Zhicheng Zuo, Kesavan Babu, Chhandosee Ganguly, Ashwini Zolekar, Sydney Newsom, Rakhi Rajan, Yu-Chieh Wang, Jin Liu
Publikováno v:
CRISPR J
The RNA-guided Cas9 nuclease from Streptococcus pyogenes has become an important gene-editing tool. However, its intrinsic off-target activity is a major challenge for biomedical applications. Distinct from some reported engineering strategies that s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01ec049393171d074703e5d39bcbf62d
https://europepmc.org/articles/PMC9271410/
https://europepmc.org/articles/PMC9271410/