Zobrazeno 1 - 8
of 8
pro vyhledávání: '"Rajan Hollmann"'
Publikováno v:
Bioprocess and Biosystems Engineering. 30:47-59
Fluxes of central carbon metabolism [glycolysis, pentose phosphate pathway (PPP), tricarboxylic acid cycle (TCA cycle), biomass formation] were determined for several Bacillus megaterium strains (DSM319, WH320, WH323, MS941) in C- and N-limited chemo
Autor:
Dieter Jahn, Rebekka Biedendieck, Marco Malten, W.-D. Deckwer, Rajan Hollmann, Yang Yang, Wei Wang
Publikováno v:
Engineering in Life Sciences. 6:470-474
The production of biocatalysts is usually carried out in a few well-characterized microorganisms like E. coli or, in the case of pharmaceuticals, in animal cell culture. Although well known as an established system, protein production in E. coli is r
Publikováno v:
Chemie Ingenieur Technik. 78:295-300
Neuere Untersuchungen haben gezeigt, dass Bacillus megaterium auch als Produktionsstamm bei der Expression von Fremd-Genen vorteilhaft eingesetzt werden kann. Gegenstand dieses Beitrags sind Ergebnisse aus dynamischen Untersuchungen des Aminosaure-St
Autor:
Rebekka Biedendieck, Rajan Hollmann, Marco Malten, Yang Yang, Dieter Jahn, Wei Wang, Wolf-Dieter Deckwer
Publikováno v:
Chemie Ingenieur Technik. 78:289-294
During the past years plasmid systems for recombinant protein production using Bacillus megaterium were developed. These systems are based on multicopy plasmids with functional elements for cloning in Escherichia coli and induced gene expression in B
Autor:
Wolf-Dieter Deckwer, Manfred Nimtz, Tobias Fürch, Dieter Jahn, Marco Malten, Rajan Hollmann, Wei Wang
Publikováno v:
Proteome Science, Vol 3, Iss 1, p 4 (2005)
Proteome Science
Proteome Science
A recombinant B. megaterium strain was used for the heterologous production of a glucosyltransferase (dextransucrase). To better understand the physiological and metabolic responses of the host cell to cultivation and induction conditions, proteomic
Publikováno v:
Biotechnology and bioengineering. 89(2)
Leuconostoc mesenteroides dextransucrase DsrS was recombinantly produced in Bacillus megaterium and exported into the growth medium. For this purpose a plasmid-based xylose-inducible gene expression system was optimized via introduction of a multiple
Autor:
Wolf-Dieter Deckwer, Rajan Hollmann
Publikováno v:
Journal of biotechnology. 111(1)
A recombinant Bacillus megaterium strain showed the ability to secrete large amounts of pyruvate (up to 27.8 g l −1 ) for growth rates larger than 0.15 h −1 . Cultivation below this growth rate avoids pyruvate formation while minimizing acetate a
Publikováno v:
Bioprocess & Biosystems Engineering; Jan2007, Vol. 30 Issue 1, p47-59, 13p