Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Rafael Tapia‐Rojo"'
Autor:
Florian Franz, Rafael Tapia-Rojo, Sabina Winograd-Katz, Rajaa Boujemaa-Paterski, Wenhong Li, Tamar Unger, Shira Albeck, Camilo Aponte-Santamaria, Sergi Garcia-Manyes, Ohad Medalia, Benjamin Geiger, Frauke Gräter
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-16 (2023)
Abstract The talin-vinculin axis is a key mechanosensing component of cellular focal adhesions. How talin and vinculin respond to forces and regulate one another remains unclear. By combining single-molecule magnetic tweezers experiments, Molecular D
Externí odkaz:
https://doaj.org/article/30b01a749e4c45a4a0ec2338d1b1bd92
Autor:
Jaime Andrés Rivas-Pardo, Yong Li, Zsolt Mártonfalvi, Rafael Tapia-Rojo, Andreas Unger, Ángel Fernández-Trasancos, Elías Herrero-Galán, Diana Velázquez-Carreras, Julio M. Fernández, Wolfgang A. Linke, Jorge Alegre-Cebollada
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
Testing mechanical forces on native molecules in natural environments remains a challenge. Here the authors engineer titin to carry a HaloTag-TEV insertion to allow analysis of dynamics under force in muscle fibers.
Externí odkaz:
https://doaj.org/article/3fc2e404086144d88118c34e7a917844
Autor:
Shubhasis Haldar, Rafael Tapia-Rojo, Edward C. Eckels, Jessica Valle-Orero, Julio M. Fernandez
Publikováno v:
Nature Communications, Vol 8, Iss 1, Pp 1-7 (2017)
Proteins fold under mechanical force during co-translational folding at the ribosome. Here, the authors use single molecule magnetic tweezers to study the influence of chaperones on protein folding and show that the ribosomal chaperone trigger factor
Externí odkaz:
https://doaj.org/article/c435eaed6d044a0d845d5ba987b97fa9
Autor:
Edward C. Eckels, Shubhasis Haldar, Rafael Tapia-Rojo, Jaime Andrés Rivas-Pardo, Julio M. Fernández
Publikováno v:
Cell Reports, Vol 27, Iss 6, Pp 1836-1847.e4 (2019)
Summary: The delivery of mechanical power, a crucial component of animal motion, is constrained by the universal compromise between the force and the velocity of its constituent molecular systems. While the mechanisms of force generation have been st
Externí odkaz:
https://doaj.org/article/29d69a552a404682bf00166e52125f7d
Publikováno v:
PLoS ONE, Vol 12, Iss 6, p e0179962 (2017)
Two variants of the two-component Lantibiotic Lichenicidin, produced by the strains B. Licheniformis VK21 and I89 (Lchα/ Lchβ and Bliα/ Bliβ peptides, respectively) have been investigated by means of 2 μs-long all-atom molecular dynamics simulat
Externí odkaz:
https://doaj.org/article/8d03e059829e4891958a5d31b9c57173
Autor:
Rafael Tapia-Rojo, Juan José Mazo, José Ángel Hernández, María Luisa Peleato, María F Fillat, Fernando Falo
Publikováno v:
PLoS Computational Biology, Vol 10, Iss 10, p e1003835 (2014)
The identification of protein binding sites in promoter sequences is a key problem to understand and control regulation in biochemistry and biotechnological processes. We use a computational method to analyze promoters from a given genome. Our approa
Externí odkaz:
https://doaj.org/article/83b0ff193901485abe134e8c1eee47d2
Autor:
Rafael Tapia-Rojo, Marc Mora, Stephanie Board, Jane Walker, Rajaa Boujemaa-Paterski, Ohad Medalia, Sergi Garcia-Manyes
Publikováno v:
Tapia-Rojo, R, Mora, M, Board, S, Walker, J, Boujemaa-Paterski, R, Medalia, O & Garcia-Manyes, S 2022, ' Enhanced statistical sampling reveals microscopic complexity in the talin mechanosensor folding energy landscape ', Nature Physics, pp. 52–60 . https://doi.org/10.1038/s41567-022-01808-4
Statistical mechanics can describe the major conformational ensembles determining the equilibrium free-energy landscape of a folding protein. The challenge is to capture the full repertoire of low-occurrence conformations separated by high kinetic ba
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f4c77d225dc74b729ca606f6a4bd4bea
Autor:
Florian Franz, Rafael Tapia-Rojo, Sabina Katz, Rajaa Boujemaa-Paterski, Wenhong Li, Tamar Unger, Shira Albeck, Camilo Aponte-Santamaría, Sergi Garcia-Manyes, Ohad Medalia, Benjamin Geiger, Frauke Gräter
The talin-vinculin axis is a key mechanosensing component of cellular focal adhesions. How talin and vinculin respond to forces and regulate one another remains unclear. By combining single molecule magnetic tweezer experiments, Molecular Dynamics si
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c98ac3639de9da025fedb0c74dd5dd9
https://doi.org/10.21203/rs.3.rs-1906540/v1
https://doi.org/10.21203/rs.3.rs-1906540/v1
Publikováno v:
Proc Natl Acad Sci U S A
Cells continually sample their mechanical environment using exquisite force sensors such as talin, whose folding status triggers mechanotransduction pathways by recruiting binding partners. Mechanical signals in biology change quickly over time and a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::098bcb3056c23c41e5e0a40aa70f81ad
https://doi.org/10.1073/pnas.2004091117
https://doi.org/10.1073/pnas.2004091117