CRISPR/dCAS9-mediated DNA demethylation screen identifies functional epigenetic determinants of colorectal cancer

Autor: Juan Ramón Tejedor, Alfonso Peñarroya, Javier Gancedo-Verdejo, Pablo Santamarina-Ojeda, Raúl F. Pérez, Sara López-Tamargo, Ana Díez-Borge, Juan J. Alba-Linares, Nerea González-del-Rey, Rocío G. Urdinguio, Cristina Mangas, Annalisa Roberti, Virginia López, Teresa Morales-Ruiz, Rafael R. Ariza, Teresa Roldán-Arjona, Mónica Meijón, Luis Valledor, María Jesús Cañal, Daniel Fernández-Martínez, María Fernández-Hevia, Paula Jiménez-Fonseca, Luis J. García-Flórez, Agustín F. Fernández, Mario F. Fraga

Publikováno v: Clinical Epigenetics, Vol 15, Iss 1, Pp 1-17 (2023)

Abstract Background Promoter hypermethylation of tumour suppressor genes is frequently observed during the malignant transformation of colorectal cancer (CRC). However, whether this epigenetic mechanism is functional in cancer or is a mere consequenc

Externí odkaz: https://doaj.org/article/95cb022c2afa458ba4a52e0c37ea095b

DNA methylation reprogramming of human cancer cells by expression of a plant 5-methylcytosine DNA glycosylase

Autor: Teresa Morales-Ruiz, María Victoria García-Ortiz, Iván Devesa-Guerra, Laura Raya-Ruiz, Juan R. Tejedor, Gustavo F. Bayón, Marta I. Sierra, Mario F. Fraga, Rafael R. Ariza, Teresa Roldán-Arjona

Publikováno v: Epigenetics, Vol 13, Iss 1, Pp 95-107 (2018)

Patterns of DNA methylation, an important epigenetic modification involved in gene silencing and development, are disrupted in cancer cells. Understanding the functional significance of aberrant methylation in tumors remains challenging, due in part

Externí odkaz: https://doaj.org/article/716ad87d66bd4c03897422f5f20273f6

Targeted DNA demethylation in human cells by fusion of a plant 5-methylcytosine DNA glycosylase to a sequence-specific DNA binding domain

Autor: Jara Teresa Parrilla-Doblas, Rafael R. Ariza, Teresa Roldán-Arjona

Publikováno v: Epigenetics, Vol 12, Iss 4, Pp 296-303 (2017)

DNA methylation is a crucial epigenetic mark associated to gene silencing, and its targeted removal is a major goal of epigenetic editing. In animal cells, DNA demethylation involves iterative 5mC oxidation by TET enzymes followed by replication-depe

Externí odkaz: https://doaj.org/article/cb89ea0cd7e1402093012c5e15b73bae

Active DNA Demethylation in Plants

Autor: Jara Teresa Parrilla-Doblas, Teresa Roldán-Arjona, Rafael R. Ariza, Dolores Córdoba-Cañero

Publikováno v: International Journal of Molecular Sciences, Vol 20, Iss 19, p 4683 (2019)

Methylation of cytosine (5-meC) is a critical epigenetic modification in many eukaryotes, and genomic DNA methylation landscapes are dynamically regulated by opposed methylation and demethylation processes. Plants are unique in possessing a mechanism

Externí odkaz: https://doaj.org/article/30d8337ba1484aca89cba30d47507d6e

Complementary Functions of Plant AP Endonucleases and AP Lyases during DNA Repair of Abasic Sites Arising from C:G Base Pairs

Autor: Dolores Córdoba-Cañero, Marina Jordano-Raya, M Dolores Moreno-Recio, Cristina Beltrán-Melero, Teresa Roldán-Arjona, Rafael R. Ariza, M Isabel Martínez-Macías

Publikováno v: International Journal of Molecular Sciences 22(16), 8763 (2021)
Helvia. Repositorio Institucional de la Universidad de Córdoba
instname
International Journal of Molecular Sciences, Vol 22, Iss 8763, p 8763 (2021)
International Journal of Molecular Sciences
Volume 22
Issue 16

Abasic (apurinic/apyrimidinic, AP) sites are ubiquitous DNA lesions arising from spontaneous base loss and excision of damaged bases. They may be processed either by AP endonucleases or AP lyases, but the relative roles of these two classes of enzyme

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e647eec2cc6de5e3b9051779bc81f995
http://hdl.handle.net/10396/21703