Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Raf, Lemmens"'
Publikováno v:
Journal of Chromatography A. 1057:115-124
An integrated process for purifying a 6.1 kilo base pair (kbp) plasmid from a clarified Escherichia coli cell lysate based on an ultra/diafiltration step combined with polymer/polymer aqueous two-phase system and a new type of chromatography is descr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a980ff14bc6a6f15c805876ac4cf7fa
https://doi.org/10.1016/j.chroma.2004.09.060
https://doi.org/10.1016/j.chroma.2004.09.060
Publikováno v:
Journal of Chromatography A. 1038:131-140
We have prepared a new type of anion exchanger, which effectively discriminates between RNA and plasmid DNA. The material is based on a Sephacryl S-500 HR matrix provided with quartenary amine anion-exchange groups. A distinguishing feature of the be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7a395d9d78f8916bf8e71bf53c0bfd8
https://doi.org/10.1016/j.chroma.2004.03.050
https://doi.org/10.1016/j.chroma.2004.03.050
Publikováno v:
Journal of Biotechnology. 109:193-199
Supercoiled plasmid DNA was selectively purified from its open circular form by thiophilic interaction chromatography, performed in the presence of high concentrations of water-structuring salts. To identify optimal conditions for purification, vario
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::881241333f1afbbf0c4387eb4c1bd3d6
https://doi.org/10.1016/j.jbiotec.2003.10.036
https://doi.org/10.1016/j.jbiotec.2003.10.036
Publikováno v:
The Journal of Gene Medicine. 6:S54-S66
The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in vaccines since the advantageous safety concerns associated with non-viral over viral vector
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1613edee2676a642dc36105e83eb6388
https://doi.org/10.1002/jgm.512
https://doi.org/10.1002/jgm.512
Publikováno v:
Journal of Chromatography B. 784:291-300
Separation of the different plasmid isoforms is a major challenge in purifying plasmid DNA. We describe a new type of biochemical interaction that occurs in the presence of high concentrations of lyotropic salt and results in the selective adsorption
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06fc3e6217218843c292fed3298b97c3
https://doi.org/10.1016/s1570-0232(02)00805-x
https://doi.org/10.1016/s1570-0232(02)00805-x
Publikováno v:
European Journal of Biochemistry. 267:4106-4114
In this study, we have investigated the distribution of the enzyme nucleoside triphosphate diphosphohydrolase-1 (NTPDase1; EC 3.6.1.5) in a subset of pig tissues by biochemical activity and Western blotting with antibodies against porcine NTPDase1. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a89564a9b2568078a44d344ddca1eb8f
https://doi.org/10.1046/j.1432-1327.2000.01462.x
https://doi.org/10.1046/j.1432-1327.2000.01462.x
Publikováno v:
Journal of Biological Chemistry. 275:5640-5647
We have identified and characterized a novel ATP diphosphohydrolase (ATPDase) with features of E-type ATPases from porcine liver. Immunoblotting with a specific monoclonal antibody to this ectoenzyme revealed high expression in liver with lesser amou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7b1e8aaa79b98f31e36c7ecf097aee17
https://doi.org/10.1074/jbc.275.8.5640
https://doi.org/10.1074/jbc.275.8.5640
Publikováno v:
The Histochemical Journal. 27:555-564
1. Using the incorporation of [methyl-3H]thymidine as a proliferation marker, the effects of various nucleosides and nucleotides on endothelial LLC-MK2 cells were studied. We found that ATP, ADP, AMP and adenosine in concentrations of 10 microM or hi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5916634fb77bbea2bc2269e0cfa05eaa
https://doi.org/10.1007/bf00174328
https://doi.org/10.1007/bf00174328
Publikováno v:
European Journal of Biochemistry. 227:150-160
The protein responsible for the (Ca2+ or Mg2+)-ATPase activity in brush-border membranes from pig kidney tubular cells was characterized to distinguish this enzyme from the N-ethylmaleimide-sensitive Mg2+-ATPase, also present in renal brush borders.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::523dbfee543b1bddb09abe46fbaeb131
https://doi.org/10.1111/j.1432-1033.1995.tb20371.x
https://doi.org/10.1111/j.1432-1033.1995.tb20371.x
Autor:
Raf Lemmens, Joseph D. Bruton, Mhairi Frame, Chun-Liang Shi, Masroor Ahmed, Solveig Persson-Sjögren, Brian L. Furman, Md. Shahidul Islam, Håkan Westerblad, Nigel J. Pyne
Publikováno v:
The FASEB Journal. 17:301-303
The ryanodine (RY) receptors in beta-cells amplify signals by Ca2+-induced Ca2+ release (CICR). The role of CICR in insulin secretion remains unclear in spite of the fact that caffeine is known to stimulate secretion. This effect of caffeine is attri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::59b3f8c69737c79ef0a6d9cac0422773
https://doi.org/10.1096/fj.02-0481fje
https://doi.org/10.1096/fj.02-0481fje