Výsledky vyhledávání - "Radhey Sham Manik"

Akademický článek

Activation and Inhibition of The Wnt3A Signaling Pathway in Buffalo (Bubalus bubalis) Embryonic Stem Cells: Effects of WNT3A, Bio and Dkk1

Autor: Mohammad Zandi, Syed Mohamad Shah, Musharifa Muzaffar, Manoj Kumar Singh, Prabhat Palta, Suresh Kumar Singla, Radhey Sham Manik, Manmohan Singh Chauhan

Publikováno v: International Journal of Fertility and Sterility, Vol 9, Iss 3, Pp 361-370 (2015)

Background This research studies the effects of activation and inhibition of Wnt3A signaling pathway in buffalo (Bubalus bubalis) embryonic stem (ES) cell-like cells. Materials and Methods To carry on this experimental study, the effects of activatio

Externí odkaz: https://doaj.org/article/f2dedac2bcd1402eb01bb10856ab11d7

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Production of Transgenic Handmade Cloned Goat (Capra hircus) Embryos by Targeted Integration into Rosa 26 Locus Using Transcription Activator-like Effector Nucleases

Autor: Manmohan Singh Chauhan, Ramakant Kaushik, Radhey Sham Manik, Ankur Sharma, Suresh Kumar Singla, Manoj Kumar Singh, Preeti Vats, Prabhat Palta, Nidhi Rawat, Tushar Sharma, Diksha Dua

Publikováno v: Cellular Reprogramming. 23:250-262

Transgenic goats are ideal bioreactors for the production of therapeutic proteins in their mammary glands. However, random integration of the transgene within-host genome often culminates in unstab...

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8f004be98c3e44d4fd8cc534b4bf3b41
https://doi.org/10.1089/cell.2021.0011

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Effect of basic fibroblast growth factor (FGF2) on cumulus cell expansion, in vitro embryo production and gene expression in buffalo (Bubalus bubalis)

Autor: Radhey Sham Manik, Prabhat Palta, Manmohan Singh Chauhan, Satish Kumar, Suresh Kumar Singla

Publikováno v: Reproductive Biology. 20:501-511

The present study was undertaken to evaluate the effect of different concentration of FGF2 viz. 5 ng (T1), 10 ng (T2), and 20 ng/mL (T3) on cumulus cell expansion, oocyte maturation, in vitro embryo production, total cell number (TCN) of the blastocy

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::064ea6795f80c4676fd9ba4356be3d2a
https://doi.org/10.1016/j.repbio.2020.08.003

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Production of Transgenic Handmade Cloned Goat (

Autor: Preeti, Vats, Ramakant, Kaushik, Nidhi, Rawat, Ankur, Sharma, Tushar, Sharma, Diksha, Dua, Manoj Kumar, Singh, Prabhat, Palta, Suresh Kumar, Singla, Radhey Sham, Manik, Manmohan Singh, Chauhan

Publikováno v: Cellular reprogramming. 23(4)

Transgenic goats are ideal bioreactors for the production of therapeutic proteins in their mammary glands. However, random integration of the transgene within-host genome often culminates in unstable expression and unpredictable phenotypes. Targeting

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::3011d8a40279e61fdddeca3fadb74469
https://pubmed.ncbi.nlm.nih.gov/34348041

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Buffalo SCNT embryos exhibit abnormal gene expression of ERK/MAPK pathway and DNA methylation

Autor: Radhey Sham Manik, Manmohan Singh Chauhan, Suresh Kumar Singla, Tanushri Jerath Sood, Manishi Mukesh, Disha Ashok, Prabhat Palta, Shrutika Sah

Publikováno v: Reproduction in Domestic Animals. 53:1247-1252

Inhibition of ERK/MAPK pathway has been shown to decrease DNA methylation via down-regulation of DNA methyltransferases (DNMTs) in several studies suggesting that this pathway plays an important role in regulation of DNA methylation. We examined the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66d3304b1fe86026fbb9fda01982b6ab
https://doi.org/10.1111/rda.13217

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m-carboxycinnamic acid bishydroxamide improves developmental competence, reduces apoptosis and alters epigenetic status and gene expression pattern in cloned buffalo (Bubalus bubalis ) embryos

Autor: Suresh Kumar Singla, Himanshu Agrawal, Prabhat Palta, Naresh L. Selokar, Manoj Kumar Singh, Monika Saini, Chauhan, Radhey Sham Manik

Publikováno v: Reproduction in Domestic Animals. 53:986-996

Incomplete or aberrant reprogramming of nuclear genome is one of the major problems in somatic cell nuclear transfer. In this study, we studied the effect of histone deacetylase inhibitor m-carboxycinnamic acid bishydroxamide (CBHA) on in vitro devel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::476dc26d03267fa73161ece13f0f3c62
https://doi.org/10.1111/rda.13198

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Selection of Suitable Internal Control Genes for Accurate Normalization of Real-Time Quantitative PCR Data of Buffalo (Bubalus bubalis) Blastocysts Produced by SCNT and IVF

Autor: Tanushri Jerath Sood, Prabhat Palta, Ankita Sharma, Manmohan Singh Chauhan, Swati Viviyan Lagah, Suresh Kumar Singla, Manishi Mukesh, Radhey Sham Manik

Publikováno v: Cellular Reprogramming. 19:302-310

We evaluated the suitability of 10 candidate internal control genes (ICGs), belonging to different functional classes, namely ACTB, EEF1A1, GAPDH, HPRT1, HMBS, RPS15, RPS18, RPS23, SDHA, and UBC for normalizing the real-time quantitative polymerase c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cf712aa58be128bd3c3788eeafdbd6ea
https://doi.org/10.1089/cell.2017.0010

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Retinoic acid induces differentiation of buffalo (Bubalus bubalis) embryonic stem cells into germ cells

Autor: Prabhat Palta, Syed Mohmad Shah, Manmohan Singh Chauhan, Radhey Sham Manik, Suresh Kumar Singla

Publikováno v: Gene. 626:358-366

Development of precise and reproducible culture system for in vitro differentiation of embryonic stem (ES) cells into germ cells counts as a major leap forward for understanding not only the remarkable process of gametogenesis, otherwise obscured by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::820b898f2797fb79c646b31ff07a1a09
https://doi.org/10.1016/j.gene.2017.05.037

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Valproic Acid Increases Histone Acetylation and Alters Gene Expression in the Donor Cells But Does Not Improve the In Vitro Developmental Competence of Buffalo (Bubalus bubalis) Embryos Produced by Hand-Made Cloning

Autor: Suresh Kumar Singla, Prabhat Palta, Manmohan Singh Chauhan, Monika Saini, Radhey Sham Manik, Naresh L. Selokar, Himanshu Agrawal

Publikováno v: Cellular Reprogramming. 19:10-18

Use of histone deacetylase inhibitors (HDACis) is believed to improve the developmental competence and quality of cloned embryos produced. We examined the effects of treatment of buffalo fibroblasts with valproic acid (VPA), a HDACi on these cells an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f456855538570b5084f1ac2e1118876e
https://doi.org/10.1089/cell.2016.0029

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RNA sequencing and transcriptome analysis of buffalo (Bubalus bubalis) blastocysts produced by somatic cell nuclear transfer and in vitro fertilization

Autor: Radhey Sham Manik, Suresh Kumar Singla, Tanushri Jerath Sood, Manmohan Singh Chauhan, Prabhat Palta, Manishi Mukesh, Swati Viviyan Lagah

Publikováno v: Molecular reproduction and development. 86(9)

Across farm animal species, the live birth rate obtained with somatic cell nuclear transfer (SCNT) embryos is only2% compared with40% obtained with in vitro fertilization (IVF) embryos, primarily due to incomplete nuclear reprogramming which results

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06d0eaa5c7f0ac8572a7e8752c5279e6
https://pubmed.ncbi.nlm.nih.gov/31304661

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