Zobrazeno 1 - 10
of 20
pro vyhledávání: '"Radhey S. Manik"'
Autor:
Anjit Sandhu, Sushil K. Mohapatra, Manoj K. Singh, Suresh K. Singla, Manmohan S. Chauhan, Radhey S. Manik
Publikováno v:
Zygote. 31:129-139
SummaryAdult male and female Murrah buffalo fibroblast cells were used as donors for the production of embryos using handmade cloning. Both donor cells and reconstructed embryos were treated with 50 nM trichostatin-A (TSA) and 7.5 nM 5-aza-2′-deoxy
Autor:
Anjit, Sandhu, Sushil K, Mohapatra, Manoj K, Singh, Suresh K, Singla, Manmohan S, Chauhan, Radhey S, Manik
Publikováno v:
Zygote (Cambridge, England).
Adult male and female Murrah buffalo fibroblast cells were used as donors for the production of embryos using handmade cloning. Both donor cells and reconstructed embryos were treated with 50 nM trichostatin-A (TSA) and 7.5 nM 5-aza-2'-deoxycytidine
Autor:
Shrutika Sah, Radhey S. Manik, Prabhat Palta, Manmohan Singh Chauhan, Aditya K Sharma, Suresh Kumar Singla
Publikováno v:
Cellular Reprogramming. 23:304-315
We examined the effects of treatment with pulsed electromagnetic fields (PEMFs) on cumulus cells and buffalo somatic cell nuclear transfer (SCNT) embryos. PEMF treatment (30 μT for 3 hours) of cumulus cells increased (p < 0.05) the relative cell via
Publikováno v:
Reproduction in domestic animals = ZuchthygieneREFERENCES.
In this study we treated the handmade cloned (HMC) buffalo embryos with the DNA methylation inhibitors; 5-aza-2'-deoxycytidine (AzadC) or Zebularine individually after post-fusion and during in vitro culture till eighth day. The blastocysts productio
Autor:
Manoj Kumar Singh, Radhey S. Manik, Manmohan Singh Chauhan, Prabhat Palta, Suresh Kumar Singla, Tanushri Jerath Sood, Manishi Mukesh, Swati Viviyan Lagah
Publikováno v:
Cellular Reprogramming. 21:200-209
Very low birth rate and a high incidence of abnormalities in offspring born from cloned embryos, which have limited the application of cloning technology on a wide scale, are believed to be because of incomplete or aberrant nuclear reprogramming. Mic
Autor:
Prabhat Palta, Suresh Kumar Singla, Manmohan Singh Chauhan, Radhey S. Manik, Naresh L. Selokar, Monika Saini
Publikováno v:
Animal Reproduction Science. 197:1-9
The first birth of a cloned animal produced through the Handmade cloning (HMC) technique was reported more than 15 years ago in cattle. This method of somatic cell nuclear transfer (SCNT) has subsequently been evolving as a much simpler alternative t
Cloning of Buffalo, a Highly Valued Livestock Species of South and Southeast Asia: Any Achievements?
Autor:
Radhey S. Manik, Manmohan Singh Chauhan, Suresh Kumar Singla, Prabhat Palta, Naresh L. Selokar, Monika Saini
Publikováno v:
Cellular Reprogramming. 20:89-98
Buffalo (Bubalus bubalis) is a major source of milk, meat, and draught power in many developing countries in Asia. Animal cloning holds a lot of potential for fast multiplication of elite buffaloes and conservation of their valuable germplasm. Althou
Autor:
Radhey S. Manik, Manoj Kumar Singh, Sunny Dholpuria, Dharmendra Kumar Gangwar, Prabhat Palta, Suresh Kumar Singla, Sudha Saini, Shiv Kumar Yadav, V. Vinoth Khanna, Jarnail Singh, Chiranjeev Kumar Tikadar
Publikováno v:
Veterinary World, Vol 10, Iss 5, Pp 498-504 (2017)
Veterinary World
Veterinary World
Separation of X- and Y-chromosome bearing sperm has been practiced for selection of desired sex of offspring to increase the profit in livestock industries. At present, fluorescence-activated cell sorter is the only successful method for separation o
Autor:
A.K. Raja, Monika Saini, Basanti Jyotsana, A.A. Sahare, Sakshi Kaith, Manmohan Singh Chauhan, Radhey S. Manik, K. P. Singh, Pankaj K. Madheshiya, Prabhat Palta, Suresh Kumar Singla
Publikováno v:
Cellular Reprogramming. 17:160-169
This study was aimed at isolation of cells from urine and skin on the ventral part of the tails of healthy adult female buffaloes (Bubalus bubalis), an area rarely exposed to solar radiation, establishment of the cells in culture, and their use as do
Autor:
Suresh Kumar Singla, Ruchi Sharma, Aman George, Radhey S. Manik, Veena Garg, Prabhat Palta, Manmohan Singh Chauhan, K. P. Singh, Manoj Kumar Singh, Ramakant Kaushik
Publikováno v:
Cellular Reprogramming. 14:530-538
In this study, we describe the production of buffalo parthenogenetic blastocysts and subsequent isolation of parthenogenetic embryonic stem cell (PGESC)-like cells. PGESC colonies exhibited dome-shaped morphology and were clearly distinguishable from