Zobrazeno 1 - 9
of 9
pro vyhledávání: '"Radha Krishnakumar"'
Autor:
Mary Hinkle, Agnes P. Chan, Emil Lesho, Jessica DePew, Yongwook Choi, Maria Kim, Lauren M. Brinkac, Derrick E. Fouts, Radha Krishnakumar
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
In light of the ongoing antimicrobial resistance crisis, there is a need to understand the role of co-pathogens, commensals, and the local microbiome in modulating virulence and antibiotic resistance. To identify possible interactions that influence
Autor:
Jiqiang Ling, Radha Krishnakumar
Publikováno v:
FEBS Letters. 588:383-388
The addition of new and versatile chemical and biological properties to proteins pursued through incorporation of non-canonical amino acids is at present primarily achieved by stop codon suppression. However, it is critical to find new “blank” co
Autor:
Jiqiang Ling, Jesse Rinehart, John I. Glass, Laure Prat, Radha Krishnakumar, Hans R. Aerni, Dieter Söll, Chuck Merryman
Publikováno v:
ChemBioChem. 14:1967-1972
Sense codon recoding is the basis for genetic code expansion with more than two different noncanonical amino acids. It requires an unused (or rarely used) codon, and an orthogonal tRNA synthetase:tRNA pair with the complementary anticodon. The Mycopl
Autor:
Nacyra Assad-Garcia, J. Craig Venter, Zhi-Qing Qi, Prashanth P. Parmar, Ray-Yuan Chuang, Clyde A. Hutchison, Mikkel A. Algire, Christopher H. Calvey, Hamilton O. Smith, Evgeniya A. Denisova, Vladimir N. Noskov, Monzia Moodie, Daniel G. Gibson, Chuck Merryman, Carole Lartigue, Li Ma, Radha Krishnakumar, Gwynedd A. Benders, Michael G. Montague, Cynthia Andrews-Pfannkoch, Lei Young, John I. Glass, Sanjay Vashee, Thomas H. Segall-Shapiro
Publikováno v:
Science
Science, American Association for the Advancement of Science, 2010, 329 (5987), pp.52-56. ⟨10.1126/science.1190719⟩
Science, American Association for the Advancement of Science, 2010, 329 (5987), pp.52-56. ⟨10.1126/science.1190719⟩
Let There Be Life The DNA sequence information from thousands of genomes is stored digitally as ones and zeros in computer memory. Now, Gibson et al. (p. 52 , published online 20 May; see the cover; see the Policy Forum by Cho and Relman ) have broug
Publikováno v:
Journal of Bacteriology. 189:4343-4352
Salmonella enterica strains survive and propagate in macrophages by both circumventing and resisting the antibacterial effectors normally delivered to the phagosome. An important aspect of Salmonella resistance is the production of periplasmic supero
Autor:
Michael G. Montague, John I. Glass, Radha Krishnakumar, Quang Phan, Gwynedd A. Benders, Nacyra Assad-Garcia
Publikováno v:
Applied and environmental microbiology. 76(15)
Most gene knockouts in mycoplasmas are achieved through labor-intensive transposon mutagenesis. Here, we describe a method for making targeted deletions in Mycoplasma pneumoniae by use of homologous recombination. In this method, M. pneumoniae is tra
Autor:
Chuck Merryman, Jayshree Zaveri, Radha Krishnakumar, Nina Alperovich, John I. Glass, Daniel H. Haft, Daniel G. Gibson, Carissa Grose
Publikováno v:
Nucleic Acids Research
Toward achieving rapid and large scale genome modification directly in a target organism, we have developed a new genome engineering strategy that uses a combination of bioinformatics aided design, large synthetic DNA and site-specific recombinases.
Publikováno v:
Nature Biotechnology. 28:1272-1273
Two approaches for selecting oligonucleotides from complex mixtures improve the fidelity and scalability of DNA synthesis.
Autor:
Derrick E. Fouts, Granger G. Sutton, Mikeljon P. Nikolich, Derek M. Harkins, Radha Krishnakumar, Emil Lesho, Yongwook Choi, Jessica DePew, Maria Kim, Erin Beck, Xiao-Zhe Huang, Agnes P. Chan
Publikováno v:
Genome Biology
Background Infections by pan-drug resistant Acinetobacter baumannii plague military and civilian healthcare systems. Previous A. baumannii pan-genomic studies used modest sample sizes of low diversity and comparisons to a single reference genome, lim