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pro vyhledávání: '"Rachel N. Oldfield"'
Autor:
Kathryn A. Johnston, Anjum Hussain, Rachel N. Oldfield, Jesica Gonzalez, Karlo M. Lopez, Madison A. Smith
Publikováno v:
Enzyme Research, Vol 2016 (2016)
Enzyme Research
Enzyme Research
Lysyl oxidase is an important extracellular matrix enzyme that has not been fully characterized due to its low solubility. In order to circumvent the low solubility of this enzyme, three solubility tags (Nus-A, Thioredoxin (Trx), and Glutathione-S-Tr
Identification of Histidine 303 as the Catalytic Base of Lysyl Oxidase via Site-Directed Mutagenesis
Autor:
Caitlin Ghilarducci, Rachel N. Oldfield, Jeanette Limones, Kathryn A. Johnston, Karlo M. Lopez
Publikováno v:
The protein journal. 37(1)
Lysyl oxidase (LOX) is a copper-dependent amine oxidase enzyme that catalyzes the formation of crosslinkages of collagen and elastin in connective tissues by oxidative deamination of lysine. Using site-directed mutagenesis, Histidine 303 has been sho